RRC ID 12413
Author Takahashi M, Haruta M.
Title Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro.
Journal Methods Mol. Biol.
Abstract For cell replacement therapies of retinal diseases, the most-needed cells are retinal pigment epithelial cells and photoreceptor cells; lens cells are needed for replacement of the cataract. Stromal cell-derived inducing activity induces the differentiation of mouse embryonic stem (ES) cells into neural cells, including midbrain tyrosine hydroxylase-positive dopaminergic neurons, and ocular cells; the method also works with primate ES cells. We describe the methods to induce retinal pigment epithelial and lens cells from monkey ES cells using stromal cell-derived inducing activity and show the characteristics of those cells in vitro and in vivo.
Volume 330
Pages 417-29
Published 2006
DOI 10.1385/1-59745-036-7:417
PMID 16846040
MeSH Animals Behavior, Animal / physiology Cell Culture Techniques* Cell Differentiation / physiology* Cell Transplantation Cells, Cultured Embryo, Mammalian / cytology Epithelial Cells / cytology Epithelial Cells / physiology* Macaca fascicularis Mice Pigment Epithelium of Eye / cytology* Rats Stem Cells / cytology Stem Cells / physiology*
Human and Animal Cells