| RRC ID |
12418
|
| 著者 |
Fukuda J, Okamura K, Ishihara K, Mizumoto H, Nakazawa K, Ijima H, Kajiwara T, Funatsu K.
|
| タイトル |
Differentiation effects by the combination of spheroid formation and sodium butyrate treatment in human hepatoblastoma cell line (Hep G2): a possible cell source for hybrid artificial liver.
|
| ジャーナル |
Cell Transplant
|
| Abstract |
The aim of this study was to investigate the feasibility of human hepatoblastoma cell line (Hep G2), which differentiates by spheroid formation, and treatment with sodium butyrate (SB) as a cell source for hybrid artificial liver (HAL). Hep G2 spontaneously formed spheroids in polyurethane foam (PUF) within 3 days of culture and restored weak ammonia removal activity. Treatment with SB, which is a histone deacetylase inhibitor, further increased the ammonia removal activity of Hep G2 spheroids in a concentration-dependent manner. The activation of ornithine transcarbamylase--a urea cycle enzyme--was significantly related to the upregulation of ammonia removal by spheroid formation, but scarcely contributed to the further upregulation following SB treatment. In contrast with ammonia removal, treatment with SB reduced the albumin secretion of Hep G2 spheroids in a concentration-dependent manner. In the PUF-HAL module in a circulation culture, the ammonia removal rate and albumin secretion rate (per unit volume of the module) of Hep G2 spheroids treated with 5 mM SB were almost the same as those of primary porcine hepatocyte spheroids. These results suggest that simultaneous use of spheroid formation and SB treatment in Hep G2 is beneficial in enhancing the functions of human hepatocytes with potential applications in regenerative medicine and drug screening.
|
| 巻・号 |
14(10)
|
| ページ |
819-27
|
| 公開日 |
2005-1-1
|
| DOI |
10.3727/000000005783982503
|
| PMID |
16454356
|
| MeSH |
Albumins / metabolism
Ammonia / metabolism
Animals
Butyrates / pharmacology*
Cell Line, Tumor
Cell Proliferation / drug effects
Enzyme Activation / physiology
Female
Fibrinogen / metabolism
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / physiology
Hepatoblastoma / metabolism
Hepatoblastoma / pathology*
Hepatoblastoma / physiopathology
Hepatocytes / drug effects
Hepatocytes / physiology
Histone Deacetylase Inhibitors
Humans
Lactic Acid / metabolism
Liver Neoplasms / metabolism
Liver Neoplasms / pathology*
Liver Neoplasms / physiopathology
Liver, Artificial*
Ornithine Carbamoyltransferase / genetics
Ornithine Carbamoyltransferase / physiology
Polyurethanes
Reverse Transcriptase Polymerase Chain Reaction
Spheroids, Cellular / drug effects
Spheroids, Cellular / physiology*
Spheroids, Cellular / transplantation
|
| IF |
3.341
|
| 引用数 |
15
|
|
WOS 分野
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| ヒト・動物細胞 |
|
