論文 - 詳細
| RRC ID | 12431 |
|---|---|
| 著者 | Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, Nonaka K, Yamashita Y. |
| タイトル | Involvement of the JAK-STAT pathway and SOCS3 in the regulation of adiponectin-generated reactive oxygen species in murine macrophage RAW 264 cells. |
| ジャーナル | J Cell Biochem |
| Abstract |
Adiponectin is a protein hormone produced by differentiating adipocytes and has been proposed to have anti-diabetic and immunosuppressive properties. We previously reported that the globular form of adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO), followed by caspase-dependent apoptotic cell death in RAW 264 cells. Here, we demonstrate that gAd-induced ROS generation and apoptosis were diminished by suppressor of cytokine signaling 3 (SOCS3). The phosphorylation level of signal transducer and activator of transcription (STAT) 3 detected by Western blotting was highest at 20 min in gAd-treated RAW 264 cells. This phosphorylation was inhibited by AG490, a specific inhibitor of janus-activator kinase (JAK). The gAd-induced ROS and NO were reduced by administration of AG490 and Jak-2-specific siRNA in RAW 264 cells. The gAd stimulation transiently induced SOCS3 mRNA expression and protein production. We examined SOCS3-overexpressing RAW 264 cells to investigate the role of the JAK-STAT pathway in gAd-induced ROS and NO generation. SOCS3 overexpression significantly reduced both ROS and NO generation. Additionally, gAd-induced caspase activation and apoptotic cell death were reduced in SOCS3 transfectants compared with vector control transfectants. These results suggest that the JAK-STAT pathway, which can be suppressed by SOCS3 expression, is involved in gAd-induced ROS and NO generation followed by apoptotic cell death. |
| 巻・号 | 111(3) |
| ページ | 597-606 |
| 公開日 | 2010-10-15 |
| DOI | 10.1002/jcb.22745 |
| PMID | 20564237 |
| MeSH | Adiponectin / metabolism* Animals Apoptosis Cell Line Janus Kinases / metabolism* Macrophages / metabolism* Mice Nitric Oxide / metabolism Phosphorylation RNA, Messenger / analysis Reactive Oxygen Species / metabolism* STAT Transcription Factors / metabolism Signal Transduction Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins / biosynthesis Suppressor of Cytokine Signaling Proteins / genetics Suppressor of Cytokine Signaling Proteins / metabolism* |
| IF | 4.237 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |