| RRC ID |
12435
|
| Author |
Asaka-Amano Y, Takiguchi Y, Yatomi M, Kurosu K, Kasahara Y, Tanabe N, Tatsumi K, Kuriyama T.
|
| Title |
Effect of treatment schedule on the interaction of Cisplatin and radiation in human lung cancer cells.
|
| Journal |
Radiat Res
|
| Abstract |
This study was designed to determine the effects of the treatment schedule on the interaction between cisplatin and radiation. Cells of a human squamous cell lung cancer cell line were treated with cisplatin and radiation using three treatment protocols: 1-h exposure to cisplatin immediately followed by irradiation (A), 4-day continuous exposure to cisplatin immediately followed by irradiation (B), and 1-h exposure to cisplatin followed by irradiation after a 4-day interval (C). The interactions were assessed by isobologram, cell cycle distribution and apoptosis. The combination resulted in a additive effect in every protocol. Cell cycle accumulation at G(2)/M phase before irradiation was observed in Protocols B and C, whereas no cell cycle shift in the limited time course was noted in Protocol A. Although a 4-day continuous exposure to cisplatin and a 1-h exposure to cisplatin followed by a 4-day interval before irradiation caused significantly increased apoptosis, an additional increase in apoptosis after irradiation was not observed in Protocols B and C, whereas Protocol A showed an additional increase. Despite a cell cycle shift favoring radiation sensitivity, the drug-radiation interactions in Protocols B and C were additive, possibly because of negative effects including induction of a durable G(2)/M-phase arrest and suppression of apoptosis by cisplatin.
|
| Volume |
167(6)
|
| Pages |
637-44
|
| Published |
2007-6-1
|
| DOI |
10.1667/RR0739.1
|
| PII |
RR0739
|
| PMID |
17523845
|
| MeSH |
Antineoplastic Agents / administration & dosage
Apoptosis / drug effects
Apoptosis / radiation effects
Carcinoma, Squamous Cell / pathology*
Carcinoma, Squamous Cell / therapy*
Cell Cycle / drug effects
Cell Cycle / radiation effects
Cell Line, Tumor
Cisplatin / administration & dosage*
Dose Fractionation, Radiation
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Drug Administration Schedule
Humans
Lung Neoplasms / pathology*
Lung Neoplasms / therapy*
Radiation Dosage
Radiation Tolerance / drug effects*
Radiation-Sensitizing Agents / administration & dosage
Radiotherapy, Adjuvant
|
| IF |
2.657
|
| Times Cited |
4
|
|
WOS Category
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
BIOPHYSICS
|
| Resource |
| Human and Animal Cells |
RERF-LC-AI(RCB0444) |
