RRC ID 12440
Author Wakita T.
Title Isolation of JFH-1 strain and development of an HCV infection system.
Journal Methods Mol. Biol.
Abstract Detailed analysis of hepatitis C virus (HCV) has been hampered by the lack of an appropriate viral culture system and small animal models of infection. My group and others have recently reported the production of infectious virus after full-length HCV RNA transfection into Huh-7 cells. This system depends primarily on isolation of a JFH-1 strain from a patient with fulminant hepatitis. The JFH-1 strain belongs to genotype 2a and has high colony-formation efficiency when tested with a subgenomic replicon system. Here, I describe various protocols for isolation of the JFH-1 strain and construction of the HCV infection system. The HCV infection system contributes to our understanding of HCV virology and may permit development of novel antiviral strategies.
Volume 510
Pages 305-27
Published 2009
DOI 10.1007/978-1-59745-394-3_23
PMID 19009271
MeSH Adult Animals Cell Line, Tumor Cloning, Molecular DNA, Complementary / biosynthesis DNA, Complementary / isolation & purification DNA, Viral / genetics Genotype Hepacivirus / classification Hepacivirus / genetics Hepacivirus / isolation & purification* Hepacivirus / pathogenicity* Hepatitis C / virology Humans Male Plasmids / genetics RNA, Viral / biosynthesis RNA, Viral / isolation & purification Reverse Transcriptase Polymerase Chain Reaction Transfection Viral Core Proteins / analysis Viral Core Proteins / metabolism Virulence Virus Cultivation / methods*
Human and Animal Cells