論文 - 詳細
| RRC ID | 12448 |
|---|---|
| 著者 | Kimura M, Soeda S, Oda M, Ochiai T, Kihara T, Ono N, Shimeno H. |
| タイトル | Release of plasminogen activator inhibitor-1 from human astrocytes is regulated by intracellular ceramide. |
| ジャーナル | J Neurosci Res |
| Abstract |
The present study underscores a regulatory role of intracellular ceramide in astrocytes for the release of an extracellular serine protease, tissue-type plasminogen activator (t-PA), and its inhibitor, plasminogen activator inhibitor-1 (PAI-1). Treatment of cultured human astrocytes with N-acetylsphingosine, a cell-permeable short-chain ceramide analogue or daunorubicin that could increase intracellular ceramide via activation of ceramide synthase or sphingomyelin hydrolysis increased the release of t-PA and conversely decreased the PAI-1 release. Interestingly, treatment of the astrocytes with tumor necrosis factor (TNF)-alpha also increased the intracellular ceramide levels but caused the elevation of PAI-1 release without altering the t-PA release. These data suggest that the generation of ceramide in astrocytes is linked at least with the regulation of PAI-1 release. We also demonstrate that the suppression of PAI-1 release with daunorubicin accelerates the cell death of neuronally differentiated PC12 cells and suggest an antiapoptotic role of PAI-1 in the nervous system. |
| 巻・号 | 62(6) |
| ページ | 781-8 |
| 公開日 | 2000-12-15 |
| DOI | 10.1002/1097-4547(20001215)62:6<781::AID-JNR4>3.0.CO;2-W |
| PII | 10.1002/1097-4547(20001215)62:6<781::AID-JNR4>3.0.CO;2-W |
| PMID | 11107162 |
| MeSH | Animals Antibiotics, Antineoplastic / pharmacology Astrocytes / drug effects Astrocytes / metabolism* Cell Death / drug effects Cell Death / physiology Ceramides / metabolism* Culture Media, Conditioned / pharmacology Daunorubicin / pharmacology Enzyme Inhibitors / pharmacology Humans Neurons / drug effects Neurons / metabolism PC12 Cells Plasminogen Activator Inhibitor 1 / metabolism* Rats Sphingosine / analogs & derivatives Sphingosine / pharmacology Tissue Plasminogen Activator / drug effects Tissue Plasminogen Activator / metabolism* Tumor Necrosis Factor-alpha / pharmacology |
| 引用数 | 39 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |