RRC ID 12449
Author Ding Y, Nguyen HT, Choi EM, Bae K, Kim YH.
Title Rhusonoside A, a new megastigmane glycoside from Rhus sylvestris, increases the function of osteoblastic MC3T3-E1 cells.
Journal Planta Med.
Abstract Chemical investigation of the stems and leaves of Rhus Sylvestris afforded a new megastigmane glycoside named rhusonoside A ( 1), along with four other known compounds: dihydroquercetin ( 2), astragalin ( 3), hyperin ( 4), and kaempferol-3- O-rutinoside ( 5). Their structures were determined by a combination of spectroscopic analysis and application of the modified Mosher's method. The effect of compounds 1 - 5 on the function of osteoblastic MC3T3-E1 cells was examined by determining cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Rhusonoside A ( 1) significantly increased the function of osteoblastic MC3T3-E1 cells. Cell viability, ALP activity, and collagen synthesis were increased dose dependently, up to 155.39 %, 171.27 %, and 134.25 %, respectively, of the basal value at 10 muM ( P < 0.05). In addition, 0.1 muM of compound 1 significantly increased mineralization of MC3T3-E1 cells to 142.78 % ( P < 0.05) of the basal value.
Volume 75(2)
Pages 158-62
Published 2009-2
DOI 10.1055/s-0028-1088373
PMID 19053017
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Calcification, Physiologic / drug effects Cell Survival / drug effects Collagen / biosynthesis Cyclohexanols / chemistry Cyclohexanols / isolation & purification Cyclohexanols / pharmacology Glucosides / chemistry Glucosides / isolation & purification Glucosides / pharmacology Glycosides / chemistry Glycosides / isolation & purification Glycosides / pharmacology* Mice Molecular Structure Norisoprenoids / chemistry Norisoprenoids / isolation & purification Norisoprenoids / pharmacology* Osteoblasts / drug effects* Osteoblasts / metabolism Plant Extracts / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plant Leaves Plant Stems Rhus / chemistry*
IF 2.494
Times Cited 5
WOS Category PLANT SCIENCES INTEGRATIVE & COMPLEMENTARY MEDICINE PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL
Resource
Human and Animal Cells