論文 - 詳細
| RRC ID | 12459 |
|---|---|
| 著者 | Kim EO, Kim N, Kim TJ, Kim K, Kim TW, Kumar V, Lee KM. |
| タイトル | Unidirectional signaling triggered through 2B4 (CD244), not CD48, in murine NK cells. |
| ジャーナル | J Leukoc Biol |
| Abstract |
Engagement of 2B4 (CD244) with CD48 results in activation, costimulation, or inhibition of NK cell activities, depending on the cell types and the stage of differentiation. In vivo, 2B4+ NK cells can interact with CD48+ NK cells and also with surrounding CD48+ hematopoietic cells. Similarly, CD48+ NK cells may be triggered by adjacent 2B4+ NK cells or other hematopoietic cells expressing 2B4, e.g., monocytes, basophils, γδ T cells, etc. As CD48 was also shown to function as an activating receptor, 2B4/CD48 binding in the settings of NK-to-NK or NK-to-non-NK cell interactions may generate bidirectional signals. To address this question, we examined the consequence of CD48 or 2B4 ligation using two experimental settings: one with target (syngeneic EL4 and allogeneic P815) cells, ectopically expressing surface 2B4 or CD48, and the other with direct cross-linking with plate-bound mAb. Here, we report that ligation of CD48 with 2B4+ EL4 or 2B4+ P815 targets, in the absence of other receptor engagement, did not alter NK cell cytotoxicity or proliferation significantly. Similarly, cross-linking of NK cells with plate-bound anti-CD48 mAb in the absence or presence of a suboptimal dose of IL-2 did not modulate NK proliferation, cytotoxicity, or cytokine production. Nonetheless, 2B4 cross-linking promoted NK cell proliferation and effector functions consistently in both settings. Therefore, our results demonstrate unequivocally that CD48 on surrounding NK or non-NK cells serves primarily as a ligand to stimulate 2B4 on the adjacent NK cells in mice. |
| 巻・号 | 88(4) |
| ページ | 707-14 |
| 公開日 | 2010-10-1 |
| DOI | 10.1189/jlb.0410198 |
| PII | jlb.0410198 |
| PMID | 20647560 |
| MeSH | Animals Antigens, CD / immunology Antigens, CD / metabolism* CD48 Antigen Cell Separation Cytotoxicity, Immunologic / immunology Female Flow Cytometry Killer Cells, Natural / immunology Killer Cells, Natural / metabolism* Lymphocyte Activation / immunology* Mice Mice, Inbred C57BL Receptors, Immunologic / immunology Receptors, Immunologic / metabolism* Signal Transduction / immunology* Signaling Lymphocytic Activation Molecule Family |
| IF | 3.757 |
| 引用数 | 5 |
| WOS 分野 | HEMATOLOGY IMMUNOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |