Reference - Detail
| RRC ID | 1245 |
|---|---|
| Author | Hayashi H, Ishisaki A, Imamura T. |
| Title | Smad mediates BMP-2-induced upregulation of FGF-evoked PC12 cell differentiation. |
| Journal | FEBS Lett |
| Abstract |
We previously reported that bone morphogenetic protein (BMP)-2 augments fibroblast growth factor (FGF)-induced neuronal differentiation of PC12 cells by selectively upregulating FGF receptor (FGFR)-1 expression. Here we describe the underlying mechanism. BMP-2 activated Smad proteins in PC12 cells. Overexpression of Smad7 or Smad1, inhibitory and receptor-regulated isoforms, respectively, suppressed or enhanced BMP-2-induced upregulation of FGFR-1 expression. Smad 7 also inhibited the FGF-induced PC12 differentiation. Our findings indicate that activation of a Smad signaling pathway is required for upregulation of FGFR-1 expression by BMP-2 and for the synergistic induction of PC12 differentiation by BMP-2 and FGF. |
| Volume | 536(1-3) |
| Pages | 30-4 |
| Published | 2003-2-11 |
| DOI | 10.1016/s0014-5793(03)00005-x |
| PII | S001457930300005X |
| PMID | 12586333 |
| MeSH | Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / pharmacology* Cell Differentiation / drug effects DNA-Binding Proteins / antagonists & inhibitors DNA-Binding Proteins / physiology* Drug Synergism Fibroblast Growth Factors / pharmacology* Neurons / cytology* Neurons / drug effects Neurons / metabolism PC12 Cells Rats Receptor Protein-Tyrosine Kinases / biosynthesis Receptor, Fibroblast Growth Factor, Type 1 Receptors, Fibroblast Growth Factor / biosynthesis Signal Transduction Smad Proteins Smad1 Protein Trans-Activators / antagonists & inhibitors Trans-Activators / physiology* Transforming Growth Factor beta* Up-Regulation* |
| IF | 3.057 |
| Times Cited | 11 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |