Reference - Detail
| RRC ID | 12460 |
|---|---|
| Author | Ogita H, Nakagawa T, Lee KY, Inaoka T, Okano T, Kikkawa YS, Sakamoto T, Ito J. |
| Title | Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus. |
| Journal | ORL J Otorhinolaryngol Relat Spec |
| Abstract |
OBJECTIVE:Previous studies have demonstrated the potential of cell transplantation for regeneration of spiral ganglion neurons (SGNs). However, the effect of surgical invasion on host cochleae has yet to be evaluated. The present study investigated the efficiency and invasiveness of our surgical procedure using a fine glass pipette for injections into the cochlear modiolus. METHODS:We examined the survival of transplanted embryonic stem cell-derived neurons in the cochlear modiolus of guinea pigs. Surgical invasiveness was assessed by measurements of electrically evoked auditory brainstem responses (eABRs) and SGN densities after an injection of 5 microl of saline into the cochlear modiolus. RESULTS:All of the transplanted animals exhibited localization of transplanted cells in the cochlear modiolus. No significant alterations in the eABR thresholds or SGN densities were found following surgery. CONCLUSION:These findings indicate that our procedure is a viable method for testing the potential of transplants for SGN replacement. |
| Volume | 71(1) |
| Pages | 32-9 |
| Published | 2009-1-1 |
| DOI | 10.1159/000165915 |
| PII | 000165915 |
| PMID | 18971600 |
| MeSH | Animals Cell Count Cell Differentiation Cell Survival Cell Transplantation / methods* Cochlea / pathology Cochlea / physiopathology Cochlea / surgery* Differential Threshold Embryonic Stem Cells / pathology Embryonic Stem Cells / transplantation* Evoked Potentials, Auditory, Brain Stem Guinea Pigs Injections Minimally Invasive Surgical Procedures* Neurons / pathology* Postoperative Period Sodium Chloride / administration & dosage Spiral Ganglion / pathology Treatment Outcome |
| IF | 1.08 |
| Times Cited | 11 |
| WOS Category | OTORHINOLARYNGOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-G2/PA6(RCB1127) |