論文 - 詳細
| RRC ID | 12470 |
|---|---|
| 著者 | Ishiguro T, Tanaka K, Sakuno T, Watanabe Y. |
| タイトル | Shugoshin-PP2A counteracts casein-kinase-1-dependent cleavage of Rec8 by separase. |
| ジャーナル | Nat Cell Biol |
| Abstract |
During meiosis, the cohesin complexes that maintain sister chromatid cohesion are lost in a stepwise manner. At meiosis I the cohesin subunit Rec8 is cleaved only along the chromosome arms; until meiosis II it is protected at centromeres by the action of shugoshin (Sgo1)-protein phosphatase 2A (PP2A). Although this regulation hypothetically involves phosphorylation that is antagonized by Sgo1-PP2A, the kinase and substrate that are responsible are as yet unknown. Using a genetic screen for 'anti-shugoshin', we identify Hhp2, an orthologue of casein kinase 1delta/epsilon (CK1), as a factor required for Rec8 cleavage in fission yeast. We show that CK1, rather than a Polo-like kinase that is widely believed to do so, acts as the cohesin kinase to promote this cleavage during meiosis. Crucially, forced localization of excess Hhp2 at the pericentromeric region abrogates the ability of Sgo1-PP2A to protect centromeric Rec8. Thus, our studies prove the key notion that the balance between Rec8 phosphorylation and its dephosphorylation by Sgo1-PP2A regulates the step-wise loss of chromosomal cohesion in meiosis. |
| 巻・号 | 12(5) |
| ページ | 500-6 |
| 公開日 | 2010-5-1 |
| DOI | 10.1038/ncb2052 |
| PII | ncb2052 |
| PMID | 20383139 |
| MeSH | Casein Kinase I / physiology* Cell Cycle Proteins / metabolism* Cell Cycle Proteins / physiology* Centromere Chromatids Endopeptidases / metabolism* Hydrolysis Meiosis Phosphoproteins / metabolism* Phosphorylation Protein Phosphatase 2 / physiology* Schizosaccharomyces Schizosaccharomyces pombe Proteins / metabolism* Separase |
| IF | 20.042 |
| 引用数 | 97 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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