RRC ID |
1263
|
著者 |
Kazeto Y, Ijiri S, Matsubara H, Adachi S, Yamauchi K.
|
タイトル |
Molecular cloning and characterization of 3beta-hydroxysteroid dehydrogenase/Delta5-Delta4 isomerase cDNAs from Japanese eel ovary.
|
ジャーナル |
J Steroid Biochem Mol Biol
|
Abstract |
3beta-hydroxysteroid dehydrogenase/Delta(5)-Delta(4) isomerase (3beta-HSD) is a crucial steroidogenic enzyme which catalyzes an essential step in the biosynthesis of all classes of steroid hormones. Two closely related cDNAs, encoding Japanese eel ovarian types I and II 3beta-HSD, were cloned and characterized. Both cDNAs putatively encoded 375 amino acid residues sharing high sequence homology with those of rainbow trout (71%) and mammalian (approximately 45-50%) 3beta-HSD. Transient expression of types I and II 3beta-HSD in COS-7 cells revealed that both proteins possess 3beta-hydroxysteroid dehydrogenase as well as Delta(5)-Delta(4) isomerase activity for both pregnenolone and dehydroepiandrosterone, with the preference of pregnenolone over dehydroepiandrosterone as substrate, although the type I protein is more active than the type II. By northern blot analysis, a single band of the 3beta-HSD transcript of approximately 1.5kb in length was observed in ovarian tissue and the total transcript abundance of both 3beta-HSDs remained constant throughout ovarian development artificially induced by gonadotropin-rich salmon pituitary homogenate. This lack of change in 3beta-HSD transcript abundance during ovarian development did not correlate with the fluctuation of its enzymatic activity reported previously, which may suggest that changes in 3beta-HSD activity during ovarian development may be, in part, post-transcriptionally regulated in the Japanese eel ovary.
|
巻・号 |
85(1)
|
ページ |
49-56
|
公開日 |
2003-5-1
|
DOI |
10.1016/s0960-0760(03)00138-9
|
PII |
S0960076003001389
|
PMID |
12798356
|
MeSH |
17-Hydroxysteroid Dehydrogenases / chemistry
17-Hydroxysteroid Dehydrogenases / genetics*
17-Hydroxysteroid Dehydrogenases / metabolism
Amino Acid Sequence
Androstenedione / metabolism
Anguilla / genetics
Anguilla / growth & development
Anguilla / metabolism*
Animals
Base Sequence
Blotting, Northern
COS Cells
Chlorocebus aethiops
Cloning, Molecular
DNA, Complementary / genetics
Female
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Molecular Sequence Data
Ovary / enzymology*
Ovary / growth & development
Progesterone / metabolism
RNA / chemistry
RNA / genetics
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
|
IF |
3.813
|
引用数 |
24
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
COS-7(RCB0539) |