論文 - 詳細
RRC ID | 1264 |
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著者 | Kawase K, Shibata M, Kawashima H, Hatsuzawa K, Nagahama M, Tagaya M, Tani K. |
タイトル | Gaf-1b is an alternative splice variant of Gaf-1/Rip11. |
ジャーナル | Biochem Biophys Res Commun |
Abstract |
Gaf-1/Rip11 encoded by the clone KIAA0857 participates in endosomal recycling through the interaction with both gamma-SNAP, a member of the soluble NSF attachment protein family, and a small GTPase, Rab11. Gaf-1/Rip11 and other Rab11-interacting proteins constitute a novel protein family that is involved in the endocytic pathways. Here we report the presence of an alternative splice variant of Gaf-1/Rip11 named Gaf-1b. Gaf-1b also interacts with gamma-SNAP and is expressed ubiquitously in tissues except for liver. Subcellular fractionation analysis revealed that Gaf-1b, as well as Gaf-1/Rip11, is mainly present in the microsomal fraction. Overexpression of Gaf-1b, like that of Gaf-1/Rip11, affected the morphology of recycling endosomes. These results suggest that Gaf-1b has a similar function to Gaf-1/Rip11. |
巻・号 | 303(4) |
ページ | 1042-6 |
公開日 | 2003-4-18 |
DOI | 10.1016/s0006-291x(03)00486-8 |
PII | S0006291X03004868 |
PMID | 12684040 |
MeSH | Adaptor Proteins, Signal Transducing Alternative Splicing* Carrier Proteins / analysis Carrier Proteins / genetics* Carrier Proteins / metabolism Carrier Proteins / physiology* Cell Line Endosomes / ultrastructure HeLa Cells Humans Membrane Proteins / analysis Membrane Proteins / genetics* Membrane Proteins / metabolism Membrane Proteins / physiology* Mitochondrial Proteins* Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Trans-Activators / analysis Trans-Activators / genetics* Vesicular Transport Proteins* |
IF | 2.985 |
引用数 | 4 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | HeLa(RCB0007) |