RRC ID 12661
Author Tanaka H, Arimura Y, Yabana T, Goto A, Hosokawa M, Nagaishi K, Yamashita K, Yamamoto H, Sasaki Y, Fujimiya M, Imai K, Shinomura Y.
Title Myogenic lineage differentiated mesenchymal stem cells enhance recovery from dextran sulfate sodium-induced colitis in the rat.
Journal J Gastroenterol
Abstract BACKGROUND:Although mounting evidence implicates mesenchymal stem cells (MSCs) in intestinal tissue repair, uncertainty remains concerning the distribution, function, and fate of repopulating MSCs in recipient colonic tissues. Therefore, we investigated the role of transplanted MSCs in the repair phase of DSS colitis.
METHODS:LacZ-labeled rat MSCs were transplanted into rats with colitis induced by 4% DSS on day 2. Regular water replaced the DSS solution on day 6. Therapeutic effect was evaluated on day 9 by clinicopathologic and growth factor/cytokine expression profiles. We analyzed the Notch signaling pathway by Western blotting and characterized immunofluorescence of lacZ-labeled MSCs with confocal laser microscopy. In vivo differentiation of MSC was confirmed by transmission electron microscopy (TEM).
RESULTS:Recovery of colitis was modestly but significantly promoted by MSC transplantation due to proceeding cell cycle and inhibiting apoptosis in the epithelia. Tgfa mRNA expression increased significantly, while Notch signaling was inhibited in the colonic tissues with MSC transplantation. β-Galactosidase-positive cells, which expressed α-SMA, desmin, and vimentin, were infrequently detected in the lamina propria stroma. DSS exposure in vitro proved to be the most potent inducer for α-SMA in MSCs where TEM demonstrated myogenic lineage differentiation.
CONCLUSIONS:We found that MSCs transplantation modestly promoted the repair of DSS colitis. The donor-derived MSCs were likely reprogrammed to differentiate to myogenic lineage cells by cues from the micro milieu. Further characterization of these cells is warranted as a basis for applying cell-based therapy for inflammatory bowel disease.
Volume 46(2)
Pages 143-52
Published 2011-2-1
DOI 10.1007/s00535-010-0320-7
PMID 20848145
MeSH Actins / metabolism Analysis of Variance Animals Apoptosis Body Weight Cell Cycle Cell Differentiation Cell Lineage Colitis / chemically induced Colitis / metabolism* Colitis / pathology Colitis / therapy* Colon / metabolism* Colon / pathology Cytokines / metabolism Desmin / metabolism Dextran Sulfate Disease Models, Animal Intestinal Mucosa / metabolism Intestinal Mucosa / pathology Mesenchymal Stem Cell Transplantation* Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism RNA, Messenger / metabolism Rats Rats, Inbred Lew Signal Transduction Transforming Growth Factor alpha / metabolism Vimentin / metabolism beta-Galactosidase / metabolism
IF 6.132
Times Cited 26
Rats LEW-Tg(Gt(ROSA)26Sor-lacZ)44Jmsk(strainID=648)