RRC ID 12664
Author Zhao LC, Zhang XD, Liao SX, Gao HC, Wang HY, Lin DH.
Title A metabonomic comparison of urinary changes in Zucker and GK rats.
Journal J. Biomed. Biotechnol.
Abstract To further investigate pathogenesis and pathogenic process of type 2 diabetes mellitus (T2DM), we compared the urinary metabolic profiling of Zucker obese and Goto-kakizaki (GK) rats by NMR-based metabonomics. Principal component analysis (PCA) on urine samples of both models rats indicates markedly elevated levels of creatine/creatinine, dimethylamine, and acetoacetate, with concomitantly declined levels of citrate, 2-ketoglurarate, lactate, hippurate, and succinate compared with control rats, respectively. Simultaneously, compared with Zucker obese rats, the GK rats show decreased levels of trimethylamine, acetate, and choline, as well as increased levels of creatine/creatinine, acetoacetate, alanine, citrate, 2-ketoglutarate, succinate, lactate, and hippurate. This study demonstrates metabolic similarities between the two stages of T2DM, including reduced tricarboxylic acid (TCA) cycle and increased ketone bodies production. In addition, compared with Zucker obese rats, the GK rats have enhanced concentration of energy metabolites, which indicates energy metabolic changes produced in hyperglycemia stage more than in insulin resistance stage.
Volume 2010
Pages 431894
Published 2010
DOI 10.1155/2010/431894
PMID 20981252
PMC PMC2963802
MeSH Acids, Acyclic / urine Animals Biomarkers / urine* Creatine / urine Diabetes Mellitus, Type 2 / genetics Diabetes Mellitus, Type 2 / physiopathology* Diabetes Mellitus, Type 2 / urine* Dimethylamines / urine Disease Models, Animal* Hippurates / urine Hyperglycemia / physiopathology Hyperglycemia / urine Insulin Resistance / physiology* Ketone Bodies / biosynthesis Ketone Bodies / urine Magnetic Resonance Spectroscopy Male Metabolomics* Multivariate Analysis Obesity / metabolism Obesity / physiopathology Rats Rats, Wistar Rats, Zucker Species Specificity Taurine / urine
Rats KZ-Leprfa/Tky(strainID=32)