Reference - Detail
| RRC ID | 12667 |
|---|---|
| Author | Kashiwazaki N, Seita Y, Takizawa A, Maedomari N, Ito J, Serikawa T. |
| Title | Techniques for in vitro and in vivo fertilization in the rat. |
| Journal | Methods Mol Biol |
| Abstract |
Although in vitro and in vivo fertilization are powerful tools for restoring conserved sperm as well as stocked males in the rat, the techniques have progressively gained importance. However, the techniques are not used extensively for efficient production of rat offspring, because the techniques require a great deal of skill. This chapter describes the protocols for in vitro and in vivo fertilization in the rat. Namely, sperm collection, sperm cryopreservation, pre-incubation of sperm, and insemination (co-culture with sperm and oocytes) for in vitro fertilization and intrauterine insemination for in vivo fertilization with fresh or frozen/thawed spermatozoa are provided. |
| Volume | 597 |
| Pages | 311-22 |
| Published | 2010-1-1 |
| DOI | 10.1007/978-1-60327-389-3_22 |
| PMID | 20013243 |
| MeSH | Animals Coculture Techniques / methods Cryopreservation / methods Cryopreservation / veterinary Female Fertilization* Fertilization in Vitro / methods* Fertilization in Vitro / veterinary* Insemination, Artificial / methods* Insemination, Artificial / veterinary* Male Oocytes / cytology Rats / physiology* Rats, Wistar / physiology Semen Preservation / methods Semen Preservation / veterinary Spermatozoa / cytology |
| Times Cited | 3 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Rats | Technical Report |