論文 - 詳細
| RRC ID | 12748 |
|---|---|
| 著者 | Isaji T, Sato Y, Fukuda T, Gu J. |
| タイトル | N-glycosylation of the I-like domain of beta1 integrin is essential for beta1 integrin expression and biological function: identification of the minimal N-glycosylation requirement for alpha5beta1. |
| ジャーナル | J Biol Chem |
| Abstract |
N-Glycosylation of integrin alpha5beta1 plays a crucial role in cell spreading, cell migration, ligand binding, and dimer formation, but the detailed mechanisms by which N-glycosylation mediates these functions remain unclear. In a previous study, we showed that three potential N-glycosylation sites (alpha5S3-5) on the beta-propeller of the alpha5 subunit are essential to the functional expression of the subunit. In particular, site 5 (alpha5S5) is the most important for its expression on the cell surface. In this study, the function of the N-glycans on the integrin beta1 subunit was investigated using sequential site-directed mutagenesis to remove the combined putative N-glycosylation sites. Removal of the N-glycosylation sites on the I-like domain of the beta1 subunit (i.e. the Delta4-6 mutant) decreased both the level of expression and heterodimeric formation, resulting in inhibition of cell spreading. Interestingly, cell spreading was observed only when the beta1 subunit possessed these three N-glycosylation sites (i.e. the S4-6 mutant). Furthermore, the S4-6 mutant could form heterodimers with either alpha5S3-5 or alpha5S5 mutant of the alpha5 subunit. Taken together, the results of the present study reveal for the first time that N-glycosylation of the I-like domain of the beta1 subunit is essential to both the heterodimer formation and biological function of the subunit. Moreover, because the alpha5S3-5/beta1S4-6 mutant represents the minimal N-glycosylation required for functional expression of the beta1 subunit, it might also be useful for the study of molecular structures. |
| 巻・号 | 284(18) |
| ページ | 12207-16 |
| 公開日 | 2009-5-1 |
| DOI | 10.1074/jbc.M807920200 |
| PII | S0021-9258(20)58380-4 |
| PMID | 19261610 |
| PMC | PMC2673289 |
| MeSH | Cell Line Glycosylation Humans Integrin alpha5beta1 / genetics Integrin alpha5beta1 / metabolism* Integrin beta1 / biosynthesis* Integrin beta1 / genetics Mutagenesis, Site-Directed Mutation, Missense Protein Modification, Translational / physiology* Protein Structure, Tertiary / physiology |
| IF | 4.238 |
| 引用数 | 64 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |