RRC ID 12763
Author Sasaki M, Hasebe R, Makino Y, Suzuki T, Fukushi H, Okamoto M, Matsuda K, Taniyama H, Sawa H, Kimura T.
Title Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.
Journal Genes Cells
Abstract The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells.
Volume 16(4)
Pages 343-57
Published 2011-4-1
DOI 10.1111/j.1365-2443.2011.01491.x
PMID 21306483
PMC PMC3118799
MeSH Animals Endothelial Cells / virology Genes, MHC Class I / genetics* Genes, MHC Class I / physiology Genetic Testing Herpesviridae Infections / genetics Herpesviridae Infections / immunology Herpesviridae Infections / veterinary* Herpesvirus 1, Equid / pathogenicity* Horse Diseases / genetics Horse Diseases / immunology* Horses / immunology Mice Molecular Sequence Data NIH 3T3 Cells / virology Viral Envelope Proteins / immunology*
IF 1.655
Times Cited 27
DNA material pCAG-HIVgp (RDB04394) CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCMV-VSV-G-RSV-Rev (RDB04393) CS-RfA-CMV-mRFP1 (RDB05747) pENTR4-H1 (RDB04395) Equine brain microvascular endothelial cell cDNA library