RRC ID |
12763
|
Author |
Sasaki M, Hasebe R, Makino Y, Suzuki T, Fukushi H, Okamoto M, Matsuda K, Taniyama H, Sawa H, Kimura T.
|
Title |
Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.
|
Journal |
Genes Cells
|
Abstract |
The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells.
|
Volume |
16(4)
|
Pages |
343-57
|
Published |
2011-4-1
|
DOI |
10.1111/j.1365-2443.2011.01491.x
|
PMID |
21306483
|
PMC |
PMC3118799
|
MeSH |
Animals
Endothelial Cells / virology
Genes, MHC Class I / genetics*
Genes, MHC Class I / physiology
Genetic Testing
Herpesviridae Infections / genetics
Herpesviridae Infections / immunology
Herpesviridae Infections / veterinary*
Herpesvirus 1, Equid / pathogenicity*
Horse Diseases / genetics
Horse Diseases / immunology*
Horses / immunology
Mice
Molecular Sequence Data
NIH 3T3 Cells / virology
Viral Envelope Proteins / immunology*
|
IF |
1.655
|
Times Cited |
27
|
WOS Category
|
GENETICS & HEREDITY
CELL BIOLOGY
|
Resource |
DNA material |
pCAG-HIVgp (RDB04394) CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCMV-VSV-G-RSV-Rev (RDB04393) CS-RfA-CMV-mRFP1 (RDB05747) pENTR4-H1 (RDB04395)
Equine brain microvascular endothelial cell cDNA library |