論文 - 詳細
| RRC ID | 1278 |
|---|---|
| 著者 | Maeda K, Nishiyama C, Tokura T, Akizawa Y, Nishiyama M, Ogawa H, Okumura K, Ra C. |
| タイトル | Regulation of cell type-specific mouse Fc epsilon RI beta-chain gene expression by GATA-1 via four GATA motifs in the promoter. |
| ジャーナル | J Immunol |
| Abstract |
The FcR beta-chain, a subunit of two related multisubunit receptor complexes, the FcepsilonRI and FcgammaRIII, amplifies the mast cell response and is necessary for the cell surface expression of FcepsilonRI in mouse. The transient reporter assay indicated that -69/+4 region is required for cell type-specific transcriptional regulation of mouse beta-chain gene. EMSA using Abs against transcription factors or competitive oligonucleotides demonstrated that -58/-40 region (containing overlapping three GATA-1 sites, -53/-48, -46/-51, and -42/-47) and -31/-26 region (containing one GATA-1 site) are recognized by GATA-1. The promoter activity of beta-chain was decreased by nucleotide replacements of the GATA-1 sites in mouse mast cell line PT18. Furthermore, exogenously produced GATA-1 up-regulated the promoter activity in CV-1 cells, which are negative in the beta-chain production and the up-regulation was apparently suppressed by GATA-1 site mutations. These results indicate that cell type-specific transcription of mouse beta-chain gene is regulated by GATA-1. |
| 巻・号 | 170(1) |
| ページ | 334-40 |
| 公開日 | 2003-1-1 |
| DOI | 10.4049/jimmunol.170.1.334 |
| PMID | 12496417 |
| MeSH | Amino Acid Motifs / genetics Amino Acid Motifs / immunology Animals Binding Sites / genetics Binding Sites / immunology Cell Line DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Gene Expression Regulation / immunology* Leukemia L5178 Mast Cells / immunology* Mast Cells / metabolism* Mice Molecular Sequence Data Promoter Regions, Genetic / immunology* Protein Binding / genetics Protein Binding / immunology Receptors, IgE / biosynthesis* Receptors, IgE / genetics* Repetitive Sequences, Nucleic Acid / immunology* Transcription Factors / genetics Transcription Factors / metabolism Transcription Factors / physiology* Transcriptional Activation / immunology Tumor Cells, Cultured |
| IF | 4.886 |
| 引用数 | 51 |
| WOS 分野 | IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | CV-1(RCB0160) |