Reference - Detail
|Author||Fujishiro J, Kawana H, Inoue S, Shimizu H, Yoshino H, Hakamata Y, Kaneko T, Murakami T, Hashizume K, Kobayashi E.|
|Title||Efficiency of adenovirus-mediated gene transduction in heart grafts in rats.|
AIM:We determined the characteristics of transgene expression of heart grafts following ex vivo gene transfer using an adenovirus vector. Transgene expression was assessed periodically in the same animals by a non-invasive bioimaging system.
METHODS:Rat heterotopic heart transplantation was performed in a syngenic combination. We infused 1 x 10(9) plaque-forming units of adenovirus vectors containing firefly luciferase gene into the heart graft via the coronary artery, with preservation at 4 degrees C and transplanted into the cervix of the recipient. Transgene expression was periodically visualized and quantified by a noninvasive bioimaging system without sacrificing experimental animals.
RESULTS:Transgene expression in the graft peaked at day 7 and then fell gradually. Transgene expression was also observed in the recipient liver.
CONCLUSIONS:We have determined the time course of transgene expression in the heart graft. This constitutes important information about ex vivo gene therapy for heart grafts.
|MeSH||Adenoviridae / immunology Animals Genes, Reporter Heart Transplantation / immunology* Luciferases / analysis Luciferases / genetics Male Rats Rats, Inbred Lew Transduction, Genetic / methods* Transplantation, Isogeneic / immunology|
|WOS Category||SURGERY TRANSPLANTATION IMMUNOLOGY|