論文 - 詳細
| RRC ID | 129 |
|---|---|
| 著者 | Fujishiro J, Kawana H, Inoue S, Shimizu H, Yoshino H, Hakamata Y, Kaneko T, Murakami T, Hashizume K, Kobayashi E. |
| タイトル | Efficiency of adenovirus-mediated gene transduction in heart grafts in rats. |
| ジャーナル | Transplant Proc |
| Abstract |
AIM:We determined the characteristics of transgene expression of heart grafts following ex vivo gene transfer using an adenovirus vector. Transgene expression was assessed periodically in the same animals by a non-invasive bioimaging system. METHODS:Rat heterotopic heart transplantation was performed in a syngenic combination. We infused 1 x 10(9) plaque-forming units of adenovirus vectors containing firefly luciferase gene into the heart graft via the coronary artery, with preservation at 4 degrees C and transplanted into the cervix of the recipient. Transgene expression was periodically visualized and quantified by a noninvasive bioimaging system without sacrificing experimental animals. RESULTS:Transgene expression in the graft peaked at day 7 and then fell gradually. Transgene expression was also observed in the recipient liver. CONCLUSIONS:We have determined the time course of transgene expression in the heart graft. This constitutes important information about ex vivo gene therapy for heart grafts. |
| 巻・号 | 37(1) |
| ページ | 67-9 |
| 公開日 | 2005-1-1 |
| DOI | 10.1016/j.transproceed.2004.12.295 |
| PII | S0041-1345(04)01753-1 |
| PMID | 15808549 |
| MeSH | Adenoviridae / immunology Animals Genes, Reporter Heart Transplantation / immunology* Luciferases / analysis Luciferases / genetics Male Rats Rats, Inbred Lew Transduction, Genetic / methods* Transplantation, Isogeneic / immunology |
| IF | 0.784 |
| 引用数 | 3 |
| WOS 分野 | SURGERY TRANSPLANTATION IMMUNOLOGY |
| リソース情報 | |
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