Reference - Detail
|Author||Shimada S, Iwabuchi K, Watano K, Shimizu H, Yamada H, Minakami H, Onoé K.|
|Title||Expression of allograft inflammatory factor-1 in mouse uterus and poly(I:C)-induced fetal resorption.|
|Journal||Am. J. Reprod. Immunol.|
PROBLEM:To investigate whether the allograft inflammatory factor-1 (AIF-1) is expressed and plays a role in the reproductive system.
METHOD OF STUDY:AIF-1 expression was examined in uteri of non-pregnant and pregnant mice by Northern blot analysis, RT-PCR and immunohistochemistry.
RESULTS:The expression of AIF-1 varied during the estrous cycle with a peak at estrus. After the insemination, the expression of AIF-1 mRNA diminished gradually and again increased in the pre-implantation or implantation period in allogeneic or syngeneic pregnancy, respectively. Enhanced expressions of AIF-1, tumor necrosis factor-alpha (TNF-alpha) and nitric oxide synthase 2 (NOS2) mRNA were observed in the embryos of resorption-prone pregnancy injected with poly(I:C).
CONCLUSIONS:This study demonstrated for the first time that AIF-1 was expressed in uterus. The expression level was associated with the population size of macrophage and varied during the estrous cycle and the pregnancy period. The augmented expression of AIF-I with concomitant expressions of TNF-alpha and NOS2 mRNA in poly(I:C)-injected mice suggests a correlation between AIF-1 production and fetal resorption.
|MeSH||Animals Blotting, Northern Calcium-Binding Proteins / analysis* Calcium-Binding Proteins / genetics Embryo, Mammalian / chemistry Epithelial Cells / chemistry Estrous Cycle Female Fetal Resorption / chemically induced Fetal Resorption / genetics Fetal Resorption / metabolism* Gene Expression Regulation / drug effects Immunohistochemistry / methods Interferon-gamma / genetics Macrophages / chemistry Macrophages / cytology Macrophages / metabolism Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Mice, Inbred DBA Microfilament Proteins Placenta / chemistry Poly I-C / pharmacology* Pregnancy RNA, Messenger / genetics RNA, Messenger / metabolism Tumor Necrosis Factor-alpha / genetics Uterus / chemistry* Uterus / metabolism|
|WOS Category||REPRODUCTIVE BIOLOGY IMMUNOLOGY|
|Mice||AIF-1 Tgm Line Y(RBRC00471)|