論文 - 詳細
| RRC ID | 1313 |
|---|---|
| 著者 | Hattori M, Suzuki AZ, Higo T, Miyauchi H, Michikawa T, Nakamura T, Inoue T, Mikoshiba K. |
| タイトル | Distinct roles of inositol 1,4,5-trisphosphate receptor types 1 and 3 in Ca2+ signaling. |
| ジャーナル | J Biol Chem |
| Abstract |
Three subtypes of inositol 1,4,5-trisphosphate receptor (IP(3)R1, IP(3)R2, and IP(3)R3) Ca(2+) release channel share basic properties but differ in terms of regulation. To what extent they contribute to complex Ca(2+) signaling, such as Ca(2+) oscillations, remains largely unknown. Here we show that HeLa cells express comparable amounts of IP(3)R1 and IP(3)R3, but knockdown by RNA interference of each subtype results in dramatically distinct Ca(2+) signaling patterns. Knockdown of IP(3)R1 significantly decreases total Ca(2+) signals and terminates Ca(2+) oscillations. Conversely, knockdown of IP(3)R3 leads to more robust and long lasting Ca(2+) oscillations than in controls. Effects of IP(3)R3 knockdown are surprisingly similar in COS-7 cells that predominantly (>90% of total IP(3)R) express IP(3)R3, suggesting that IP(3)R3 functions as an anti-Ca(2+)-oscillatory unit without contributing to peak amplitude of Ca(2+) signals, irrespective of its relative expression level. Therefore, differential expression of the IP(3)R subtype is critical for various forms of Ca(2+) signaling, and, particularly, IP(3)R1 and IP(3)R3 have opposite roles in generating Ca(2+) oscillations. |
| 巻・号 | 279(12) |
| ページ | 11967-75 |
| 公開日 | 2004-3-19 |
| DOI | 10.1074/jbc.M311456200 |
| PII | S0021-9258(19)64415-7 |
| PMID | 14707143 |
| MeSH | Amino Acid Sequence Animals Calcium Channels / chemistry Calcium Channels / physiology* Cell Line Humans Inositol 1,4,5-Trisphosphate Receptors Molecular Sequence Data Protein Isoforms / physiology* RNA Interference Receptors, Cytoplasmic and Nuclear / chemistry Receptors, Cytoplasmic and Nuclear / physiology* Sequence Homology, Amino Acid Signal Transduction* |
| IF | 4.238 |
| 引用数 | 94 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) COS-7(RCB0539) |