RRC ID 1323
Author Yazawa S, Okada M, Ono M, Fujii S, Okuda Y, Shibata I, Kida H.
Title Experimental dual infection of pigs with an H1N1 swine influenza virus (A/Sw/Hok/2/81) and Mycoplasma hyopneumoniae.
Journal Vet. Microbiol.
Abstract Dual infection of pigs with swine influenza virus (SIV) and Mycoplasma hyopneumoniae was carried out to compare the clinical and pathological effects of dual infection in caesarian derived and colostrums deprived (CDCD) pigs, with that of a single infection with M. hyopneumoniae. In Experiment 1, 40-day-old CDCD pigs were inoculated only with SIV (A/Sw/Hok/2/81, H1N1). The virus was isolated from nasal swabs for 5-6 days. None of these pigs showed clinical signs of infection throughout the experimental period. These results suggested that this strain can infect pigs but is only slightly pathogenic when it is inoculated singly to a CDCD pig. In Experiment 2, 60-day-old CDCD pigs were inoculated with M. hyopneumoniae and then were inoculated with SIV (A/Sw/Hok/2/81) at 1 week (MHYO-7d-SIV-7d group) or 3 weeks (MHYO-21d-SIV-7d group) after M. hyopneumoniae inoculation. Macroscopically, dark red-to-purple lung lesions were observed in all of pigs at 14 or 28 days post-inoculation. Percentages of dark red-to-purple lung lesions in dual infection groups (MHYO-7d-SIV-7d group: 18.7 +/- 4.2%, MHYO-21d-SIV-7d group: 23.0 +/- 8.0%) were significantly (P < 0.05) increased compared to those of each control group in which pigs were inoculated only with M. hyopneumoniae (MHYO-14d group: 4.7 +/- 2.9%, MHYO-28 group: 3.3 +/- 2.4%). Microscopically, bronchial epithelial lesions (epithelial disruption, degeneration, hyperplasia and formation of microabscess) were frequently observed in dark red-to-purple lung lesions of only the dual infection groups. These results demonstrate that the lung lesion of pigs inoculated with M. hyopneumoniae and SIV is more severe than that of pigs inoculated only with M. hyopneumoniae.
Volume 98(3-4)
Pages 221-8
Published 2004-3-5
DOI 10.1016/j.vetmic.2003.11.005
PII S0378113503003730
PMID 15036530
MeSH Animals Antibodies, Viral / blood Body Temperature Bronchoalveolar Lavage Fluid / microbiology Germ-Free Life Hemagglutination Inhibition Tests / veterinary Histocytochemistry / veterinary Influenza A Virus, H1N1 Subtype* Influenza A virus / growth & development* Lung / microbiology Lung / pathology Mycoplasma hyopneumoniae / growth & development* Orthomyxoviridae Infections / metabolism Orthomyxoviridae Infections / pathology Orthomyxoviridae Infections / veterinary* Orthomyxoviridae Infections / virology Pneumonia of Swine, Mycoplasmal / microbiology* Pneumonia of Swine, Mycoplasmal / virology* Swine Swine Diseases / microbiology* Swine Diseases / pathology Swine Diseases / virology* Virus Shedding
IF 2.791
Times Cited 26
WOS Category VETERINARY SCIENCES MICROBIOLOGY
Resource
Human and Animal Cells MDCK