RRC ID 1330
Author Terakawa M, Ogura M, Sato S, Wakisaka H, Ashida H, Uenoyama M, Masaki Y, Obara M.
Title Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.
Journal Opt Lett
Abstract Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency.
Volume 29(11)
Pages 1227-9
Published 2004-6-1
DOI 10.1364/ol.29.001227
PMID 15209255
MeSH Animals Cell Membrane / metabolism* Cell Membrane / radiation effects* Cell Membrane Permeability / radiation effects* Gene Transfer Techniques Genetic Therapy / methods Green Fluorescent Proteins Lasers* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mice NIH 3T3 Cells Plasmids / administration & dosage* Plasmids / genetics Plasmids / pharmacokinetics* Stress, Mechanical Temperature Transfection / methods*
IF 3.714
Times Cited 52
WOS Category OPTICS
Resource
Human and Animal Cells NIH3T3-3