論文 - 詳細
| RRC ID | 1336 |
|---|---|
| 著者 | Tajima K, Demachi A, Ito Y, Nishida K, Akatsuka Y, Tsujimura K, Kuwano H, Mitsudomi T, Takahashi T, Kuzushima K. |
| タイトル | Identification of an epitope from the epithelial cell adhesion molecule eliciting HLA-A*2402-restricted cytotoxic T-lymphocyte responses. |
| ジャーナル | Tissue Antigens |
| Abstract |
Because the epithelial cell adhesion molecule (Ep-CAM) is expressed in almost all carcinomas and human leucocyte antigen (HLA)-A*2402 is the most common allele in many ethnic groups, including Japanese, the identification of peptide sequences, which elicit HLA-A*2402-restricted Ep-CAM-specific cytotoxic T-lymphocyte (CTL) responses, would facilitate specific immunotherapy for various histological types of carcinomas. An epitope was identified through the following steps: (i) computer-based epitope prediction from the amino acid sequence of Ep-CAM, (ii) major histocompatibility complex (MHC) stabilization assay to determine the affinity of the predicted peptide with HLA-A*2402 molecules, (iii) stimulation of CD8+ T cells with peptide-pulsed dendritic cells and (iv) testing the CTL specificity by means of enzyme-linked immunospot (ELISPOT) assays, CTL assays and MHC/peptide-tetramer staining. Peripheral CD8+ T cells of four of five healthy donors after three rounds of stimulation with the peptide Ep-CAM173-181 (RYQLDPKFI) secreted interferon-gamma in ELISPOT assays when exposed to the peptide. A CTL clone specific to the peptide efficiently lysed Ep-CAM-expressing cancer cell lines in an HLA-A*2402-restricted fashion. Endogenous processing and presentation of the peptide in a lung cancer cell line were confirmed by means of cold target inhibition assays. The CTL clone was also lytic to normal bronchial epithelial cells but to a lesser extent at low effector: target ratios. All these data suggest that the peptide-specific CTL responses may play some roles both in anti-cancer and autoimmune reactions. The peptide should prove useful to study anti-Ep-CAM CTL responses among population possessing HLA-A*2402. |
| 巻・号 | 64(6) |
| ページ | 650-9 |
| 公開日 | 2004-12-1 |
| DOI | 10.1111/j.1399-0039.2004.00329.x |
| PII | TAN329 |
| PMID | 15546337 |
| MeSH | Biomarkers, Tumor / immunology CD8-Positive T-Lymphocytes / immunology* Cell Adhesion Colonic Neoplasms / immunology Colonic Neoplasms / metabolism Colonic Neoplasms / pathology Dendritic Cells Epithelial Cells / immunology* Epitopes / immunology* HLA-A Antigens / immunology* HLA-A24 Antigen Humans Interferon-gamma / metabolism Lung Neoplasms / immunology* Lung Neoplasms / metabolism Lung Neoplasms / pathology Peptide Fragments / immunology Stomach Neoplasms / immunology Stomach Neoplasms / metabolism Stomach Neoplasms / pathology T-Lymphocytes, Cytotoxic / immunology* Tumor Cells, Cultured |
| IF | 1.348 |
| 引用数 | 10 |
| WOS 分野 | PATHOLOGY IMMUNOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LC-1/sq(RCB0455) |