論文 - 詳細
| RRC ID | 1349 |
|---|---|
| 著者 | Hirose H, Arasaki K, Dohmae N, Takio K, Hatsuzawa K, Nagahama M, Tani K, Yamamoto A, Tohyama M, Tagaya M. |
| タイトル | Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi. |
| ジャーナル | EMBO J |
| Abstract |
ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in the endoplasmic reticulum as well as in the cytosol during interphase, and forms a subcomplex with RINT-1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking. Like conventional syntaxin-binding proteins, ZW10, RINT-1 and p31 dissociated from syntaxin 18 upon Mg(2+)-ATP treatment in the presence of NSF and alpha-SNAP, whereas the subcomplex was not disassembled. Overexpression, microinjection and knockdown experiments revealed that ZW10 is involved in membrane trafficking between the endoplasmic reticulum and Golgi. The present results disclose an unexpected role for a spindle checkpoint protein, ZW10, during interphase. |
| 巻・号 | 23(6) |
| ページ | 1267-78 |
| 公開日 | 2004-3-24 |
| DOI | 10.1038/sj.emboj.7600135 |
| PII | 7600135 |
| PMID | 15029241 |
| PMC | PMC381410 |
| MeSH | Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Cycle Proteins / metabolism Cell Line Dynactin Complex Dyneins / metabolism Endoplasmic Reticulum / genetics Endoplasmic Reticulum / metabolism* Gene Expression Regulation Golgi Apparatus / genetics Golgi Apparatus / metabolism* Humans Interphase Intracellular Membranes / metabolism* Intracellular Signaling Peptides and Proteins Membrane Proteins / genetics Membrane Proteins / metabolism Microtubule-Associated Proteins / metabolism Nuclear Proteins Protein Binding Protein Transport Qa-SNARE Proteins Two-Hybrid System Techniques |
| IF | 9.889 |
| 引用数 | 132 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |