| RRC ID |
1353
|
| Author |
Jiang B, Hattori N, Liu B, Nakayama Y, Kitagawa K, Sumita K, Inagaki C.
|
| Title |
Expression and roles of Cl- channel ClC-5 in cell cycles of myeloid cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
ClC-5 is a chloride channel known to be expressed in the kidney. We previously reported that ClC-5 mRNA was also strongly expressed in immature human myeloid cell line (HL-60), but weakly expressed in mature neutrophils. To clarify the underlying mechanisms, we examined the relationship between ClC-5 expression and cell cycle. Dimethyl sulfoxide treatment of HL-60 that causes differentiation with G0/G1 cell cycle arrest decreased the expression of ClC-5 mRNA. Cell sorting and synchronization experiments revealed that ClC-5 mRNA expression was high in S and G2/M phases and low in G0/G1 phase. ClC-5 antisense oligonucleotide suppressed proliferation of HL-60 cells with a decrease in ClC-5 protein expression, probably due to G2 arrest. These results suggest that cell cycle-dependent expression of ClC-5 has a role in cell cycle progression in myeloid cells.
|
| Volume |
317(1)
|
| Pages |
192-7
|
| Published |
2004-4-23
|
| DOI |
10.1016/j.bbrc.2004.03.036
|
| PII |
S0006291X04005157
|
| PMID |
15047167
|
| MeSH |
Cell Cycle / drug effects
Cell Cycle / physiology
Cell Differentiation
Chloride Channels / biosynthesis
Chloride Channels / genetics
Chloride Channels / metabolism*
DNA / analysis
Dimethyl Sulfoxide / pharmacology
Gene Expression / drug effects
HL-60 Cells
Humans
Interphase
Myeloid Cells / cytology*
Myeloid Cells / metabolism*
Neutrophils / metabolism
Oligonucleotides, Antisense / pharmacology
RNA, Messenger / biosynthesis
|
| IF |
2.985
|
| Times Cited |
21
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HL60(RCB0041) |
