| RRC ID |
1362
|
| Author |
Ishihara S, Rumi MA, Kadowaki Y, Ortega-Cava CF, Yuki T, Yoshino N, Miyaoka Y, Kazumori H, Ishimura N, Amano Y, Kinoshita Y.
|
| Title |
Essential role of MD-2 in TLR4-dependent signaling during Helicobacter pylori-associated gastritis.
|
| Journal |
J Immunol
|
| Abstract |
TLR4, a member of pattern recognition receptors, is the main receptor of LPS. MD-2 physically associates with TLR4 on the cell surface and confers LPS responsiveness. Helicobacter pylori LPS is one of the major virulence factors for induction of gastritis. We demonstrated in this study the role of MD-2 in TLR4-dependent signaling in H. pylori-associated gastritis. Gastric biopsy samples collected from patients with and without H. pylori infection and four gastric cancer cell lines were used for this study. TLR-4 and MD-2 expression in biopsy specimens and the cell lines was examined by using RT-PCR. Localization of TLR-4 in histological sections was evaluated by immunohistochemistry. For in vitro functional assays, we established stable transfectants of AGS cells expressing TLR4 and MD-2. Cellular distribution of TLR4 was examined by flow cytometry. NF-kappaB activation and activation of IL-8 and MD-2 promoters were assessed by reporter gene assay. H. pylori infection up-regulated the TLR4 and MD-2 expression in gastric mucosa. TLR4 staining was observed predominantly in epithelial cells, located in both the cytoplasm and at the apical surface. MD-2 transfection in AGS cells markedly increased cell surface expression of TLR4 and augmented the activation of NF-kappaB and IL-8 promoter upon stimulation with H. pylori LPS. Live H. pylori also stimulated transcriptional activation of MD-2. This study revealed that MD-2 expression is elevated in gastric epithelial cells during H. pylori infection, suggesting that the TLR4/MD-2 system is a potent receptor complex involved in the response to H. pylori LPS in the stomach.
|
| Volume |
173(2)
|
| Pages |
1406-16
|
| Published |
2004-7-15
|
| DOI |
10.4049/jimmunol.173.2.1406
|
| PMID |
15240737
|
| MeSH |
Adult
Antigens, Surface / genetics
Antigens, Surface / metabolism*
Female
Gastritis / immunology
Gastritis / metabolism*
Gastritis / microbiology
Genes, Reporter
Helicobacter Infections / immunology*
Helicobacter Infections / metabolism
Helicobacter pylori / immunology
Helicobacter pylori / metabolism
Humans
Interleukin-8 / genetics
Lipopolysaccharides / metabolism
Lymphocyte Antigen 96
Male
Membrane Glycoproteins / genetics
Membrane Glycoproteins / metabolism*
Middle Aged
NF-kappa B / metabolism
Promoter Regions, Genetic
RNA, Messenger / metabolism
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Signal Transduction / physiology*
Toll-Like Receptor 4
Toll-Like Receptors
|
| IF |
4.886
|
| Times Cited |
134
|
|
WOS Category
|
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
MKN7(RCB0999)
MKN45(RCB1001)
MKN28(RCB1000) |
