論文 - 詳細
| RRC ID | 1364 |
|---|---|
| 著者 | Jiang B, Hattori N, Liu B, Nakayama Y, Kitagawa K, Inagaki C. |
| タイトル | Suppression of cell proliferation with induction of p21 by Cl(-) channel blockers in human leukemic cells. |
| ジャーナル | Eur J Pharmacol |
| Abstract |
The existence of Cl(-) channels in lymphocytes and neutrophils has been increasingly recognized, but the biological functions are not yet clear. We examined the effects of Cl(-) channel blockers on the cell proliferation and the cell cycle of human leukemic cell lines. The growth of leukemic cells was suppressed most efficiently by NPPB (5-nitro-2-(3-phenylpropylamino) benzoic acid), partially by 9-AC (9-anthracenecarboxylic acid) and tamoxifen, but not by stilbene compounds. NPPB increased the G0/G1 population and induced the expression of p21, one of the critical molecules for G1/S checkpoint. Antisense oligonucleotide for a NPPB-sensitive and stilbene-insensitive Cl(-) channel, ClC-2, sufficiently suppressed the ClC-2 protein synthesis, but did not affect the growth of leukemic cells. These findings suggest that NPPB-sensitive and stilbene-insensitive Cl(-) channels other than ClC-2 play important roles in cell cycles and cell proliferation of human leukemic cells. |
| 巻・号 | 488(1-3) |
| ページ | 27-34 |
| 公開日 | 2004-3-19 |
| DOI | 10.1016/j.ejphar.2004.02.008 |
| PII | S0014299904001402 |
| PMID | 15044032 |
| MeSH | Blotting, Northern Blotting, Western CLC-2 Chloride Channels Cell Cycle / drug effects Cell Division / drug effects Chloride Channels / antagonists & inhibitors* Chloride Channels / drug effects Chloride Channels / metabolism Flow Cytometry HL-60 Cells Humans Jurkat Cells Leukemia / metabolism* Nitrobenzoates / pharmacology Oligonucleotides, Antisense / pharmacology Oncogene Protein p21(ras) / biosynthesis* RNA / biosynthesis Transfection Tumor Cells, Cultured |
| IF | 3.263 |
| 引用数 | 26 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |