| RRC ID |
1364
|
| 著者 |
Jiang B, Hattori N, Liu B, Nakayama Y, Kitagawa K, Inagaki C.
|
| タイトル |
Suppression of cell proliferation with induction of p21 by Cl(-) channel blockers in human leukemic cells.
|
| ジャーナル |
Eur J Pharmacol
|
| Abstract |
The existence of Cl(-) channels in lymphocytes and neutrophils has been increasingly recognized, but the biological functions are not yet clear. We examined the effects of Cl(-) channel blockers on the cell proliferation and the cell cycle of human leukemic cell lines. The growth of leukemic cells was suppressed most efficiently by NPPB (5-nitro-2-(3-phenylpropylamino) benzoic acid), partially by 9-AC (9-anthracenecarboxylic acid) and tamoxifen, but not by stilbene compounds. NPPB increased the G0/G1 population and induced the expression of p21, one of the critical molecules for G1/S checkpoint. Antisense oligonucleotide for a NPPB-sensitive and stilbene-insensitive Cl(-) channel, ClC-2, sufficiently suppressed the ClC-2 protein synthesis, but did not affect the growth of leukemic cells. These findings suggest that NPPB-sensitive and stilbene-insensitive Cl(-) channels other than ClC-2 play important roles in cell cycles and cell proliferation of human leukemic cells.
|
| 巻・号 |
488(1-3)
|
| ページ |
27-34
|
| 公開日 |
2004-3-19
|
| DOI |
10.1016/j.ejphar.2004.02.008
|
| PII |
S0014299904001402
|
| PMID |
15044032
|
| MeSH |
Blotting, Northern
Blotting, Western
CLC-2 Chloride Channels
Cell Cycle / drug effects
Cell Division / drug effects
Chloride Channels / antagonists & inhibitors*
Chloride Channels / drug effects
Chloride Channels / metabolism
Flow Cytometry
HL-60 Cells
Humans
Jurkat Cells
Leukemia / metabolism*
Nitrobenzoates / pharmacology
Oligonucleotides, Antisense / pharmacology
Oncogene Protein p21(ras) / biosynthesis*
RNA / biosynthesis
Transfection
Tumor Cells, Cultured
|
| IF |
3.263
|
| 引用数 |
26
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
