論文 - 詳細
| RRC ID | 1373 |
|---|---|
| 著者 | Feng X, Chuhjo T, Sugimori C, Kotani T, Lu X, Takami A, Takamatsu H, Yamazaki H, Nakao S. |
| タイトル | Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells. |
| ジャーナル | Blood |
| Abstract |
To identify candidate antigens in aplastic anemia (AA), we screened proteins derived from a leukemia cell line with serum of an AA patient and identified diazepam-binding inhibitor-related protein 1 (DRS-1). Enzyme-linked immunosorbent assay (ELISA) revealed high titers of anti-DRS-1 antibodies (DRS-1 Abs) in 27 (38.0%) of 71 AA patients displaying increased paroxysmal nocturnal hemoglobinuria (PNH)-type cells (PNH(+)), 2 (6.3%) of 32 PNH(-) AA patients, 5 (38.5%) of 13 PNH(+) myelodysplastic syndrome (MDS) patients, and none of 42 PNH(-) MDS patients. DRS-1 gene was abundantly expressed in myeloid leukemia cell lines and in CD34(+) cells derived from healthy individuals. Stimulation of T cells from an AA patient displaying high DRS-1 Abs with a putative CD4(+) T-cell epitope (amino acid residues [aa's] 191-204) presented by HLA-DR15, which overlapped with a hot spot (aa's 173-198) of DRS-1 Ab epitopes, gave rise to T cells cytotoxic for L cells (murine fibroblasts) that were transfected with DRB1*1501 and DRS-1. Enzyme-linked immunospot assay demonstrated increased frequency of T-cell precursors specific to the DRS-1 peptide in other HLA-DR15(+) AA patients displaying high DRS-1 Ab titers. These findings indicate that DRS-1 may serve as an autoantigen eliciting immune attack against hematopoietic stem cells in a subset of AA patients characterized by increased PNH-type cells. |
| 巻・号 | 104(8) |
| ページ | 2425-31 |
| 公開日 | 2004-10-15 |
| DOI | 10.1182/blood-2004-05-1839 |
| PII | S0006-4971(20)43304-X |
| PMID | 15217832 |
| MeSH | Anemia, Aplastic / blood Anemia, Aplastic / immunology* Anemia, Aplastic / metabolism Anemia, Aplastic / pathology* Animals Antibodies / immunology Autoantigens / blood Autoantigens / immunology* Autoantigens / metabolism Cell Differentiation Cell Line DNA, Complementary / genetics Diazepam Binding Inhibitor / blood Diazepam Binding Inhibitor / immunology* Diazepam Binding Inhibitor / metabolism Enzyme-Linked Immunosorbent Assay Epitopes / immunology Gene Expression Regulation Hemoglobinuria, Paroxysmal / immunology* Hemoglobinuria, Paroxysmal / metabolism Hemoglobinuria, Paroxysmal / pathology* Humans Mice Myelodysplastic Syndromes / immunology Myelodysplastic Syndromes / metabolism T-Lymphocytes / cytology T-Lymphocytes / immunology |
| IF | 17.794 |
| 引用数 | 49 |
| WOS 分野 | HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) KU812(RCB0495) U-937 DE-4(RCB0435) MOLT-4(RCB0206) |