| RRC ID |
1378
|
| 著者 |
Nakane T, Satoh T, Inada Y, Nakayama J, Itoh F, Chiba S.
|
| タイトル |
Molecular cloning and expression of HRLRRP, a novel heart-restricted leucine-rich repeat protein.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We isolated a novel leucine-rich repeat protein (LRRP) cDNA from E13 mouse embryos by the in silico approach. The cDNA encoded a protein of 274 amino acids having 7 leucine-rich repeat motifs at the center of the protein. An in vitro transcription/translation study showed that the cDNA coded for a peptide of approximately 31kDa. Northern blot analysis suggested that the mRNA of this novel LRRP was expressed only in the heart, although RT-PCR indicated slight expression in skeletal muscle as well. The transcripts of this gene and Nkx-2.5/Csx were detected in the early stage of cardiac differentiation of P19CL6 embryonal carcinoma cells treated with 1% dimethyl sulfoxide. The fusion protein made between it and GFP was detected at a high level in mitochondria and a low level in the nuclei of COS7 cells. The nuclei of the adult mouse heart were strongly stained with the antibody raised against the synthetic peptide of the protein. Therefore, we designated the gene as heart-restricted leucine-rich repeat protein (HRLRRP) and assume that mouse HRLRRP may play important roles in cardiac development and/or cardiac function.
|
| 巻・号 |
314(4)
|
| ページ |
1086-92
|
| 公開日 |
2004-2-20
|
| DOI |
10.1016/j.bbrc.2003.12.202
|
| PII |
S0006291X04000117
|
| PMID |
14751244
|
| MeSH |
Amino Acid Sequence
Animals
Cell Line
Cloning, Molecular
DNA, Complementary
Mice
Molecular Sequence Data
Muscle Proteins / chemistry
Muscle Proteins / genetics*
Myocardium / metabolism*
Protein Biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Transcription, Genetic
|
| IF |
2.985
|
| 引用数 |
11
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
P19.CL6(RCB1539)
COS-7(RCB0539) |
