Reference - Detail
RRC ID | 1382 |
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Author | Watanabe H, Seino T, Sato Y. |
Title | Antibodies to triosephosphate isomerase in patients with neuropsychiatric lupus. |
Journal | Biochem Biophys Res Commun |
Abstract |
Sera from patients with neuropsychiatric lupus (NP lupus) were screened for antibodies to mouse choroid plexus cell line ECPC-4 by Western blotting. A 29-kDa protein band detected in NP lupus sera was identified as triosephosphate isomerase (TPI). Using Western blotting with TPI, TPI was confirmed as the reactive molecule in sera (6 of 14 samples) and in cerebrospinal fluids (1 of 2 samples) of patients with NP lupus. Enzyme-linked immunosorbent assay with TPI showed that the serum anti-TPI antibody index of 89.8 (SD, 70.1) in NP lupus group was significantly higher than in systemic lupus erythematosus without NP manifestations, 34.6 (29.6); scleroderma, 38.2 (39.9); polymyositis/dermatomyositis, 42.1 (51.5); and control, 31.7 (27.4) groups (p<0.02). Sensitivity, specificity, and positive and negative likelihood ratios of serum anti-TPI antibody index for NP lupus were 42.9%, 94.7%, 8.1%, and 0.6%, respectively. These results suggest that anti-TPI antibodies are closely associated with NP lupus. |
Volume | 321(4) |
Pages | 949-53 |
Published | 2004-9-3 |
DOI | 10.1016/j.bbrc.2004.07.051 |
PII | S0006-291X(04)01519-0 |
PMID | 15358119 |
MeSH | Animals Autoantibodies / blood* Autoantibodies / cerebrospinal fluid Autoantigens / chemistry Autoantigens / isolation & purification Cell Line Humans In Vitro Techniques Lupus Erythematosus, Systemic / complications Lupus Erythematosus, Systemic / enzymology* Lupus Erythematosus, Systemic / immunology* Mental Disorders / enzymology* Mental Disorders / etiology Mental Disorders / immunology* Mice Nervous System Diseases / enzymology* Nervous System Diseases / etiology Nervous System Diseases / immunology* Rabbits Triose-Phosphate Isomerase / immunology* |
IF | 2.985 |
Times Cited | 21 |
WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells | ECPC-4(RCB1287) |