RRC ID 1388
著者 Sekiguchi T, Iida H, Fukumura J, Nishimoto T.
タイトル Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation.
ジャーナル Exp Cell Res
Abstract We investigated the function of DDX3Y, the Y chromosome AZFa region encoding a putative DEAD-box RNA helicase protein, the loss of which results in oligozoospermia or azoospermia in humans. The human DDX3Y amino acid sequence is similar to that of the X chromosome gene DDX3X (91.7% homology). Here we report that human Y- and X-encoded DEAD box RNA helicase proteins DDX3Y and DDX3X are interchangeable and have an essential function: both proteins rescued a temperature-sensitive mutant hamster cell line (tsET24) that was otherwise incapable of growth at a nonpermissive temperature. Mouse homologues Ddx3y and D1Pas1-PL10 also rescued the mutant cell line at a nonpermissive temperature. In situ hybridization revealed that Ddx3x mRNA was expressed in almost every cell in mouse testis, suggesting that Ddx3x is involved in spermatogenesis. A comparative study of DDX3X and DDX3Y was performed to determine the significance of DDX3Y for cell growth and spermatogenesis. Both DDX3X and DDX3Y promoter DNAs produced a similar degree of transcription in vivo, whereas deletion studies of the promoter DNAs indicated that these genes are differentially regulated. DDX3Y, similar to DDX3X, shuttles between the nucleus and cytoplasm in a crm1-dependent manner.
巻・号 300(1)
ページ 213-22
公開日 2004-10-15
DOI 10.1016/j.yexcr.2004.07.005
PII S0014-4827(04)00392-1
PMID 15383328
MeSH Active Transport, Cell Nucleus / genetics Animals Cell Line Chromosomes, Human, Y / genetics* Cricetinae DEAD-box RNA Helicases Female Genes, Regulator / genetics HeLa Cells Humans Karyopherins / genetics Karyopherins / metabolism Male Mice Minor Histocompatibility Antigens Molecular Sequence Data Mutation / genetics* Promoter Regions, Genetic / genetics Protein Isoforms / genetics Protein Isoforms / metabolism Proteins / genetics* Proteins / metabolism* RNA / genetics RNA / metabolism* RNA Helicases / genetics RNA Helicases / metabolism Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism Sequence Homology, Nucleic Acid Spermatocytes / enzymology Spermatogenesis / genetics Temperature Transcription, Genetic / genetics
IF 3.383
引用数 53
WOS 分野 ONCOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 NEC8(RCB0489) NEC14(RCB0490)