RRC ID |
1388
|
著者 |
Sekiguchi T, Iida H, Fukumura J, Nishimoto T.
|
タイトル |
Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation.
|
ジャーナル |
Exp Cell Res
|
Abstract |
We investigated the function of DDX3Y, the Y chromosome AZFa region encoding a putative DEAD-box RNA helicase protein, the loss of which results in oligozoospermia or azoospermia in humans. The human DDX3Y amino acid sequence is similar to that of the X chromosome gene DDX3X (91.7% homology). Here we report that human Y- and X-encoded DEAD box RNA helicase proteins DDX3Y and DDX3X are interchangeable and have an essential function: both proteins rescued a temperature-sensitive mutant hamster cell line (tsET24) that was otherwise incapable of growth at a nonpermissive temperature. Mouse homologues Ddx3y and D1Pas1-PL10 also rescued the mutant cell line at a nonpermissive temperature. In situ hybridization revealed that Ddx3x mRNA was expressed in almost every cell in mouse testis, suggesting that Ddx3x is involved in spermatogenesis. A comparative study of DDX3X and DDX3Y was performed to determine the significance of DDX3Y for cell growth and spermatogenesis. Both DDX3X and DDX3Y promoter DNAs produced a similar degree of transcription in vivo, whereas deletion studies of the promoter DNAs indicated that these genes are differentially regulated. DDX3Y, similar to DDX3X, shuttles between the nucleus and cytoplasm in a crm1-dependent manner.
|
巻・号 |
300(1)
|
ページ |
213-22
|
公開日 |
2004-10-15
|
DOI |
10.1016/j.yexcr.2004.07.005
|
PII |
S0014-4827(04)00392-1
|
PMID |
15383328
|
MeSH |
Active Transport, Cell Nucleus / genetics
Animals
Cell Line
Chromosomes, Human, Y / genetics*
Cricetinae
DEAD-box RNA Helicases
Female
Genes, Regulator / genetics
HeLa Cells
Humans
Karyopherins / genetics
Karyopherins / metabolism
Male
Mice
Minor Histocompatibility Antigens
Molecular Sequence Data
Mutation / genetics*
Promoter Regions, Genetic / genetics
Protein Isoforms / genetics
Protein Isoforms / metabolism
Proteins / genetics*
Proteins / metabolism*
RNA / genetics
RNA / metabolism*
RNA Helicases / genetics
RNA Helicases / metabolism
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism
Sequence Homology, Nucleic Acid
Spermatocytes / enzymology
Spermatogenesis / genetics
Temperature
Transcription, Genetic / genetics
|
IF |
3.383
|
引用数 |
53
|
WOS 分野
|
ONCOLOGY
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
NEC8(RCB0489)
NEC14(RCB0490) |