論文 - 詳細
| RRC ID | 1400 |
|---|---|
| 著者 | Kushida S, Peng BG, Uchimura E, Kuang M, Huang L, Miwa M, Ohno T. |
| タイトル | A tumour vaccine of fixed tumour fragments in a controlled-release vehicle with cytokines for therapy of hepatoma in mice. |
| ジャーナル | Dig Liver Dis |
| Abstract |
BACKGROUND:Cytokines can be strong potentiators for a tumour vaccine, but they have very short life in vivo when administered as a solution. AIMS:To evaluate the slow release of interleukin 2 from a cytokine-vehicle in vitro and in vivo and to evaluate the anti-tumour activity of a new tumour vaccine in vivo. METHODS:The tumour vaccine was composed of formalin-fixed Hepa 1-6 hepatoma tissue fragments, tuberculin and a lipid based vehicle containing granulocyte-macrophage colony-stimulating factor and interleukin 2. The quantity of interleukin 2 release from the cytokine-vehicle in vitro and in vivo was determined by a proliferation assay with CTLL-2 cell line. Hepa 1-6 hepatoma model system with C57BL/6J mice was used to examine protective and therapeutic anti-tumour effect of the vaccine. RESULTS:Release of interleukin 2 from the cytokine-vehicle lasted 5 days in vitro and 3 days in vivo. The vaccine protected 67% of mice from a Hepa 1-6 cell challenge and had a therapeutic effect by prolonging the life span of mice bearing established Hepa 1-6 tumours of 5 mm in diameter. Of the treated mice, 20% became completely tumour-free. CONCLUSIONS:Formalin-fixed tumour fragments and cytokines in controlled-release vehicle are useful in the rational design of tumour vaccines. |
| 巻・号 | 36(7) |
| ページ | 478-85 |
| 公開日 | 2004-7-1 |
| DOI | 10.1016/j.dld.2004.02.010 |
| PII | S1590-8658(04)00129-X |
| PMID | 15285528 |
| MeSH | Animals Cancer Vaccines / administration & dosage* Delayed-Action Preparations Disease Models, Animal Interleukin-2 / administration & dosage* Liver Neoplasms, Experimental / prevention & control Liver Neoplasms, Experimental / therapy* Male Mice Mice, Inbred C57BL Pharmaceutical Vehicles |
| IF | 3.57 |
| 引用数 | 2 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Hepa 1-6(RCB1638) CTLL-2(RCB0637) |