Reference - Detail
| RRC ID | 1402 |
|---|---|
| Author | Nanda K, Miyoshi N, Nakamura Y, Shimoji Y, Tamura Y, Nishikawa Y, Uenakai K, Kohno H, Tanaka T. |
| Title | Extract of vinegar "Kurosu" from unpolished rice inhibits the proliferation of human cancer cells. |
| Journal | J Exp Clin Cancer Res |
| Abstract |
The effects of the ethyl acetate extract of "Kurosu" (EK), Japanese traditional vinegar from unpolished rice, on the proliferation of a variety of human cancer cell lines were investigated by using the alamar blue assay. Cancer cell lines included colon adenocarcinoma (Caco-2), lung carcinoma (A549), breast adenocarcinoma (MCF-7), bladder carcinoma (5637), and prostate carcinoma (LNCaP) cells. EK inhibited the proliferation of all tested cell lines in a dose-dependent manner, with inhibition mostly pronounced in Caco-2 cells (up to 62% inhibition at a dose level of 0.025%). Flow cytometry of EK-treated Caco-2 cells showed a decrease in cell number in the G2/M phase and an increase in the sub-G1 phase (apoptotic). In addition, DNA fragmentation was detected in Caco-2 cells cultured with EK by immunostaining. RT-PCR analysis revealed p21 mRNA expression was induced in EK-treated Caco-2 cells. Moreover, PARP cleavage was promoted in EK-treated Caco-2 cells. These results suggest that EK causes G0/G1 arrest through p21 induction and, thus, is a potential apoptosis inducer in Caco-2 cells. |
| Volume | 23(1) |
| Pages | 69-75 |
| Published | 2004-3-1 |
| PMID | 15149153 |
| MeSH | Acetic Acid / chemistry* Antineoplastic Agents / pharmacology* Apoptosis Blotting, Western Caco-2 Cells Cell Division Cell Line, Tumor DNA / metabolism DNA Fragmentation Dose-Response Relationship, Drug Flow Cytometry Humans Immunohistochemistry Oryza Oxazines / pharmacology Plant Extracts RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Time Factors Xanthenes / pharmacology rho GTP-Binding Proteins / metabolism |
| IF | 7.068 |
| Times Cited | 40 |
| WOS Category | ONCOLOGY |
| Altmetric score |
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| The most frequently cited source | Google+ |
| Total number of mentions | 36 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CACO-2(RCB0988) 5637(RCB1191) A549(RCB0098) |