論文 - 詳細
| RRC ID | 1411 |
|---|---|
| 著者 | Watanabe K, Terada K, Jinriki T, Sato J. |
| タイトル | Effect of insulin on cephalexin uptake and transepithelial transport in the human intestinal cell line Caco-2. |
| ジャーナル | Eur J Pharm Sci |
| Abstract |
We investigated whether cephalexin transport in Caco-2 cells is regulated by insulin. After the insulin pretreatment, cephalexin uptake, and transport as well as PEPT1 mRNA and protein expression in the cells were measured. Cephalexin uptake was significantly increased by the insulin pretreatment. Insulin significantly increased cephalexin saturable uptake, but had no significant effect on the non-saturable one. PEPT1 protein expression on the apical membrane, but not PEPT1 mRNA expression, was increased by the insulin pretreatment. The enhancement of cephalexin uptake by the insulin pretreatment was inhibited by genistein, a tyrosine kinase inhibitor, and colchicine, an agent that disrupts protein translocation. Apical-to-basolateral transport of cephalexin has increased by the insulin pretreatment at the apical side and long-term insulin pretreatment at the basolateral side. It is considered that insulin mainly binds to its receptor on the apical and basolateral membranes, thereby promoting PEPT1 translocation from the intracellular pool to the apical membrane surface; consequently, PEPT1 protein expression on the apical membrane is increased. |
| 巻・号 | 21(1) |
| ページ | 87-95 |
| 公開日 | 2004-1-1 |
| DOI | 10.1016/j.ejps.2003.10.002 |
| PII | S0928098703002744 |
| PMID | 14706815 |
| MeSH | Biological Transport / drug effects Biological Transport / physiology Caco-2 Cells Cephalexin / metabolism* Dose-Response Relationship, Drug Humans Insulin / metabolism* Insulin / pharmacology Intestinal Absorption / drug effects Intestinal Absorption / physiology* Intestinal Mucosa / drug effects Intestinal Mucosa / metabolism* |
| IF | 3.616 |
| 引用数 | 12 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |