論文 - 詳細
| RRC ID | 1432 |
|---|---|
| 著者 | Hayashida Y, Honda K, Idogawa M, Ino Y, Ono M, Tsuchida A, Aoki T, Hirohashi S, Yamada T. |
| タイトル | E-cadherin regulates the association between beta-catenin and actinin-4. |
| ジャーナル | Cancer Res |
| Abstract |
The E-cadherin/catenin system acts as an invasion suppressor of epithelial malignancies. This invasion suppressive activity seems be mediated not only by the cell adhesive activity of E-cadherin but by other undetermined signaling pathways elicited by beta-catenin. In fact, cancer cells that have infiltrated the stroma reduce the expression of E-cadherin and accumulate beta-catenin. We attempted to identify the alternative partner proteins that make complexes with beta-catenin in the absence of E-cadherin. An approximately 100-kDa protein was constantly coimmunoprecipitated with beta-catenin from SW480 colorectal cancer cells, which lack the expression of E-cadherin, and was identified as actinin-4 by mass spectrometry. Transfection of E-cadherin cDNA suppressed the association between beta-catenin and actinin-4. Inhibition of E-cadherin by RNA interference transferred the beta-catenin and actinin-4 proteins into the membrane protrusions of DLD-1 cells. Immunofluorescence histochemistry of clinical colorectal cancer specimens showed that the beta-catenin and actinin-4 proteins were colocalized in colorectal cancer cells infiltrating the stroma. We reported previously that overexpression of actinin-4 induces cell motility and specifically promotes lymph node metastasis by colorectal cancer. The association between beta-catenin and actinin-4 and its regulation by E-cadherin may represent a novel molecular link connecting cell adhesion and motility. Shutting down the signals mediating this association may be worth considering as a therapeutic approach to cancer invasion and metastasis. |
| 巻・号 | 65(19) |
| ページ | 8836-45 |
| 公開日 | 2005-10-1 |
| DOI | 10.1158/0008-5472.CAN-05-0718 |
| PII | 65/19/8836 |
| PMID | 16204054 |
| MeSH | Actinin / metabolism* Actins / metabolism Amino Acid Sequence Cadherins / biosynthesis Cadherins / genetics Cadherins / metabolism* Cell Line, Tumor Cell Movement / physiology Cell Nucleus / metabolism Colorectal Neoplasms / genetics Colorectal Neoplasms / metabolism Colorectal Neoplasms / pathology Humans Laminin Microfilament Proteins / metabolism* Molecular Sequence Data Neoplasm Invasiveness Neoplasm Metastasis Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism Pancreatic Neoplasms / pathology Stromal Cells / pathology Transfection beta Catenin / biosynthesis beta Catenin / metabolism* |
| IF | 9.727 |
| 引用数 | 87 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COLO-320(RCB1193) |