RRC ID 1434
著者 Hata K, Nishimura R, Ueda M, Ikeda F, Matsubara T, Ichida F, Hisada K, Nokubi T, Yamaguchi A, Yoneda T.
タイトル A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.
ジャーナル Mol Cell Biol
Abstract Although both osteoblasts and adipocytes have a common origin, i.e., mesenchymal cells, the molecular mechanisms that define the direction of two different lineages are presently unknown. In this study, we investigated the role of a transcription factor, CCAAT/enhancer binding protein beta (C/EBPbeta), and its isoform in the regulation of balance between osteoblast and adipocyte differentiation. We found that C/EBPbeta, which is induced along with osteoblast differentiation, promotes the differentiation of mesenchymal cells into an osteoblast lineage in cooperation with Runx2, an essential transcription factor for osteogenesis. Surprisingly, an isoform of C/EBPbeta, liver-enriched inhibitory protein (LIP), which lacks the transcriptional activation domain, stimulates transcriptional activity and the osteogenic action of Runx2, although LIP inhibits adipogenesis in a dominant-negative fashion. Furthermore, LIP physically associates with Runx2 and binds to the C/EBP binding element present in the osteocalcin gene promoter. These data indicate that LIP functions as a coactivator for Runx2 and preferentially promotes the osteoblast differentiation of mesenchymal cells. Thus, identification of a novel role of the C/EBPbeta isoform provides insight into the molecular basis of the regulation of osteoblast and adipocyte commitment.
巻・号 25(5)
ページ 1971-9
公開日 2005-3-1
DOI 10.1128/MCB.25.5.1971-1979.2005
PII 25/5/1971
PMID 15713650
PMC PMC549359
MeSH Adipocytes / cytology* Adipocytes / physiology Animals CCAAT-Enhancer-Binding Protein-beta / analysis CCAAT-Enhancer-Binding Protein-beta / genetics CCAAT-Enhancer-Binding Protein-beta / physiology* Cell Differentiation / genetics Cell Differentiation / physiology Cell Line Core Binding Factor Alpha 1 Subunit Gene Expression Mesenchymal Stem Cells / chemistry Mesenchymal Stem Cells / metabolism Mesenchymal Stem Cells / physiology* Mice Neoplasm Proteins / metabolism Neoplasm Proteins / physiology* Osteoblasts / chemistry Osteoblasts / cytology* Osteoblasts / physiology Protein Isoforms / analysis Protein Isoforms / genetics Protein Isoforms / physiology Sequence Deletion / genetics Transcription Factors / metabolism Transcription Factors / physiology*
IF 3.611
引用数 68
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 10T1/2(RCB0247) C2C12(RCB0987) ST2(RCB0224) COS-7(RCB0539) 293(RCB1637)