| RRC ID |
1436
|
| Author |
Masuda S, Murakami M, Takanezawa Y, Aoki J, Arai H, Ishikawa Y, Ishii T, Arioka M, Kudo I.
|
| Title |
Neuronal expression and neuritogenic action of group X secreted phospholipase A2.
|
| Journal |
J Biol Chem
|
| Abstract |
Although individual mammalian secreted phospholipase A(2) (sPLA(2)) enzymes exhibit unique tissue and cellular distributions, the cell type-specific functions of each enzyme remain largely unknown. In this study, we found by immunohistochemistry that group X sPLA(2) (sPLA(2)-X) is uniquely located in the peripheral neuronal fibers, an observation that was supported by detection of its transcript and protein in the neuronal cell line PC12 and in primary dorsal root ganglia neurons. Adenoviral expression of sPLA(2)-X in PC12 cells facilitated neurite outgrowth, particularly when combined with a suboptimal concentration of nerve growth factor. In neuronally differentiated PC12 cells, sPLA(2)-X was preferentially localized in the Golgi apparatus and growth cones, and proteolytic conversion of the proenzyme to mature enzyme mainly occurred after the secretion process. The neurite-extending ability of sPLA(2)-X depended on the production of its catalytic product, lysophosphatidylcholine. Moreover, nerve growth factor-induced neurite extension of PC12 cells was modestly but significantly attenuated by an anti-sPLA(2)-X antibody or by a small interfering RNA for sPLA(2)-X. These observations suggest the potential contribution of sPLA(2)-X to neuronal differentiation, and possibly repair, under certain conditions.
|
| Volume |
280(24)
|
| Pages |
23203-14
|
| Published |
2005-6-17
|
| DOI |
10.1074/jbc.M500985200
|
| PII |
S0021-9258(20)61495-8
|
| PMID |
15781456
|
| MeSH |
Adenoviridae / metabolism
Animals
Blotting, Northern
Catalysis
Cell Differentiation
Cell Line
Chromatography, Thin Layer
DNA, Complementary / metabolism
Ganglia, Spinal / metabolism
Golgi Apparatus / metabolism
Humans
Immunoblotting
Immunohistochemistry
Lysophosphatidylcholines / metabolism
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Nerve Growth Factor
Neurons / enzymology*
Neurons / metabolism
PC12 Cells
Phospholipases A / biosynthesis*
Phospholipases A / metabolism*
Phospholipases A2
RNA, Small Interfering / metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
Spectrometry, Mass, Electrospray Ionization
Time Factors
|
| IF |
4.238
|
| Times Cited |
49
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
