| RRC ID |
1439
|
| Author |
Hassan F, Islam S, Mu MM, Ito H, Koide N, Mori I, Yoshida T, Yokochi T.
|
| Title |
Lipopolysaccharide prevents doxorubicin-induced apoptosis in RAW 264.7 macrophage cells by inhibiting p53 activation.
|
| Journal |
Mol Cancer Res
|
| Abstract |
The effect of lipopolysaccharide on doxorubicin-induced cell death was studied by using mouse RAW 264.7 macrophage cells. Pretreatment with lipopolysaccharide at 10 ng/mL prevented doxorubicin-induced cell death and the inhibition was roughly dependent on the concentration of lipopolysaccharide. Posttreatment with lipopolysaccharide for 1 hour also prevented doxorubicin-induced cell death. Lipopolysaccharide inhibited DNA fragmentation and caspase-3 activation in doxorubicin-treated RAW 264.7 cells, suggesting the prevention of doxorubicin-induced apoptosis. Lipopolysaccharide did not significantly inhibit doxorubicin-induced DNA damage detected by single-cell gel electrophoresis (comet) assay. Lipopolysaccharide definitely inhibited the stabilization and nuclear translocation of p53 in doxorubicin-treated RAW 264.7 cells. Lipopolysaccharide, as well as being an inhibitor of p53, abolished doxorubicin-induced apoptosis. Therefore, p53 was suggested to play a pivotal role in the prevention of doxorubicin-induced apoptosis in RAW 264.7 cells by lipopolysaccharide.
|
| Volume |
3(7)
|
| Pages |
373-9
|
| Published |
2005-7-1
|
| DOI |
10.1158/1541-7786.MCR-05-0046
|
| PII |
3/7/373
|
| PMID |
16046548
|
| MeSH |
Animals
Antibiotics, Antineoplastic / adverse effects
Antibiotics, Antineoplastic / antagonists & inhibitors*
Apoptosis / drug effects*
Cell Line
DNA Damage / drug effects
DNA Fragmentation / drug effects
Doxorubicin / adverse effects
Doxorubicin / antagonists & inhibitors*
Escherichia coli
Humans
Lipopolysaccharides / pharmacology*
Macrophages / cytology
Mice
Mice, Inbred BALB C
Tumor Suppressor Protein p53 / drug effects*
Tumor Suppressor Protein p53 / physiology
|
| IF |
4.63
|
| Times Cited |
28
|
|
WOS Category
|
ONCOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
