RRC ID 145
著者 Ochiai M, Watanabe M, Nakanishi M, Taguchi A, Sugimura T, Nakagama H.
タイトル Differential staining of dysplastic aberrant crypt foci in the colon facilitates prediction of carcinogenic potentials of chemicals in rats.
ジャーナル Cancer Lett
Abstract We developed a novel and simple method to identify dysplastic aberrant crypt foci (ACF) induced in rats by colon carcinogens more efficiently and selectively without conducting laborious histological examination, which usually requires enough time to get final diagnosis. By adding a simple decolorization process with 70% methanol after conventional 0.2% methylene blue staining, dysplastic ACF could be differentially contrasted. To examine the validity of this novel method, which we refer to as differential staining, we analyzed colonic lesions induced by three heterocyclic amines, including 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, and found that the number of dysplastic ACF detected more precisely reflected their carcinogenic potential than the total numbers of ACF.
巻・号 220(1)
ページ 67-74
公開日 2005-3-18
DOI 10.1016/j.canlet.2004.10.026
PII S0304-3835(04)00816-X
PMID 15737689
MeSH Animals Carcinogenicity Tests / methods* Carcinogens Colonic Neoplasms / chemically induced* Imidazoles Male Methylene Blue Precancerous Conditions / chemically induced* Precancerous Conditions / diagnosis Rats Rats, Inbred F344 Staining and Labeling*
IF 7.36
引用数 25
WOS 分野 ONCOLOGY
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