| RRC ID |
1453
|
| 著者 |
Shibata M, Kodani I, Osaki M, Araki K, Adachi H, Ryoke K, Ito H.
|
| タイトル |
Cyclo-oxygenase-1 and -2 expression in human oral mucosa, dysplasias and squamous cell carcinomas and their pathological significance.
|
| ジャーナル |
Oral Oncol
|
| Abstract |
Cyclo-oxygenase (COX) is a key enzyme in the conversion of arachidonic acid to prostanoids. The expression of its isoforms, COX-1 and -2 is found in many human malignancies. This study analyzed the correlation between COX expression and the pathobiological nature of human oral mucosa, dysplasias and squamous cell carcinomas (SCCs). We examined 9 specimens of normal oral epithelia, 65 lesions with dysplasias and 50 SCCs. Labeling indices (LIs) for COX-1, COX-2, Ki-67 and P53, microvessel density (MVD) and apoptotic index (AI) were evaluated using immunohistochemistry and TUNEL methods. Western blot analysis of COX-1 and -2 was performed on four human oral SCC cell lines, all of which showed expression. The LIs for COX-1 and -2 were higher for the dysplasias than the SCCs. LIs of COX-2 but not COX-1 correlated with the histological grade of dysplasia, being highest for the severe dysplasias (p < 0.05). In contrast, the COX-2 LIs as well as COX-1 were significantly (p < 0.05) inversely correlated with the histological differentiation of the SCCs. COX-2 expression was significantly correlated with LIs of COX-1 for dysplasia (p < 0.05), but not for the SCCs. In addition no significant relationship was noted between COX-2 expression and the Lis of Ki-67, P53, AI as well as MVD for the dysplasias and SCCs. The expression of COX-1 and -2 is correlated with early stage tumorigenesis and cellular differentiation of SCCs in the oral dysplasia-carcinoma sequence.
|
| 巻・号 |
41(3)
|
| ページ |
304-12
|
| 公開日 |
2005-3-1
|
| DOI |
10.1016/j.oraloncology.2004.09.009
|
| PII |
S1368-8375(04)00221-0
|
| PMID |
15743693
|
| MeSH |
Apoptosis
Biomarkers, Tumor / analysis*
Blotting, Western / methods
Carcinoma, Squamous Cell / blood supply
Carcinoma, Squamous Cell / enzymology*
Carcinoma, Squamous Cell / pathology
Cyclooxygenase 1
Cyclooxygenase 2
Humans
Immunohistochemistry / methods
In Situ Nick-End Labeling
Ki-67 Antigen / analysis
Membrane Proteins
Mouth Mucosa / enzymology*
Mouth Mucosa / pathology
Mouth Neoplasms / blood supply
Mouth Neoplasms / enzymology*
Mouth Neoplasms / pathology
Neovascularization, Pathologic
Precancerous Conditions / blood supply
Precancerous Conditions / enzymology*
Precancerous Conditions / pathology
Prostaglandin-Endoperoxide Synthases / analysis*
Statistics, Nonparametric
Tumor Suppressor Protein p53 / analysis
|
| IF |
3.979
|
| 引用数 |
81
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
SCCKN(RCB0441)
SCCTF(RCB0442) |
