論文 - 詳細
| RRC ID | 1460 |
|---|---|
| 著者 | Shimizu M, Matsuda A, Yanagisawa K, Hirota T, Akahoshi M, Inomata N, Ebe K, Tanaka K, Sugiura H, Nakashima K, Tamari M, Takahashi N, Obara K, Enomoto T, Okayama Y, Gao PS, Huang SK, Tominaga S, Ikezawa Z, Shirakawa T. |
| タイトル | Functional SNPs in the distal promoter of the ST2 gene are associated with atopic dermatitis. |
| ジャーナル | Hum Mol Genet |
| Abstract |
Atopic dermatitis (AD) is a common inflammatory skin disease associated with the local infiltration of T helper type 2 (Th2) cells. The ST2 gene encodes both membrane-bound ST2L and soluble ST2 (sST2) proteins by alternative splicing. The orphan receptor ST2L is functionally indispensable for Th2 cells. We found a significant genetic association between AD and the -26999G/A single nucleotide polymorphism (SNP) (chi2-test, raw P-value=0.000007, odds ratio 1.86) in the distal promoter region of the ST2 gene (chromosome 2q12) in a study of 452 AD patients and 636 healthy controls. The -26999A allele common among AD patients positively regulates the transcriptional activity of the ST2 gene. In addition, having at least one -26999A allele correlated with high sST2 concentrations and high total IgE levels in the sera from AD patients. Thus, the -26999A allele is correlated with an increased risk for AD. We also found that the -26999G/A SNP predominantly affected the transcriptional activity of hematopoietic cells. Immunohistochemical staining of a skin biopsy specimen from an AD patient in the acute stage showed ST2 staining in the keratinocytes as well as in the infiltrating cells in the dermal layer. Our data show that functional SNPs in the ST2 distal promoter region regulate ST2 expression which induces preferential activation of the Th2 response. Our findings will contribute to the evaluation of one of the genetic risk factors for AD. |
| 巻・号 | 14(19) |
| ページ | 2919-27 |
| 公開日 | 2005-10-1 |
| DOI | 10.1093/hmg/ddi323 |
| PII | ddi323 |
| PMID | 16118232 |
| MeSH | Alleles Case-Control Studies Dermatitis, Atopic / genetics Dermatitis, Atopic / immunology* Fibroblasts / chemistry Fibroblasts / metabolism Genes, Reporter Haplotypes Hematopoietic Stem Cells / chemistry Hematopoietic Stem Cells / metabolism Humans Interleukin-1 Receptor-Like 1 Protein Keratinocytes / chemistry Keratinocytes / metabolism Mast Cells / chemistry Mast Cells / metabolism Membrane Proteins / analysis Membrane Proteins / genetics* Membrane Proteins / metabolism Polymorphism, Single Nucleotide* Promoter Regions, Genetic / genetics* Receptors, Cell Surface Th2 Cells / immunology Transcription, Genetic |
| IF | 5.101 |
| 引用数 | 131 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HFSKF-AE-V(RCB1139) |