| RRC ID |
1467
|
| 著者 |
Suzuki T, Koyama Y, Ichikawa H, Tsushima K, Abe K, Hayakawa S, Kuruto-Niwa R, Nozawa R, Isemura M.
|
| タイトル |
1,25-Dihydroxyvitamin D3 suppresses gene expression of eukaryotic translation initiation factor 2 in human promyelocytic leukemia HL-60 cells.
|
| ジャーナル |
Cell Struct Funct
|
| Abstract |
The physiologically active metabolite of vitamin D(3), 1alpha,25-dihydroxyvitamin D(3) (DVD), is a potent inducer of cell differentiation in human myeloid leukemia cells. In the present study, we examined changes in gene expression during DVD-induced cell differentiation of promyelocytic HL-60 cells employing a DNA microarray technique. The results identified 7 up-regulated and 9 down-regulated genes with a change greater than 1.5-fold after the DVD-treatment for both 2 and 6 days. Seven of these genes were further examined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that findings obtained from the DNA microarray analysis and RT-PCR are generally comparable with each other. Gene expression of the subunits of eukaryotic translation initiation factor 2 was then examined by methods including RT-PCR and real-time PCR. The results indicated the suppression of these genes, suggesting a linkage to differentiation-associated growth inhibition of these cells.
|
| 巻・号 |
30(1)
|
| ページ |
1-6
|
| 公開日 |
2005-1-1
|
| DOI |
10.1247/csf.30.1
|
| PII |
JST.JSTAGE/csf/30.1
|
| PMID |
15951637
|
| MeSH |
Calcitriol / pharmacology*
Cell Differentiation / drug effects
Eukaryotic Initiation Factor-2 / biosynthesis
Eukaryotic Initiation Factor-2 / genetics*
Gene Expression Regulation, Leukemic / drug effects*
HL-60 Cells / drug effects
HL-60 Cells / metabolism
Humans
Neoplasm Proteins / biosynthesis
Neoplasm Proteins / genetics
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
2.429
|
| 引用数 |
12
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
