| RRC ID |
1471
|
| 著者 |
Sato K, Adachi T, Matsuo M, Tomita Y.
|
| タイトル |
Quantitative evaluation of threshold fiber strain that induces reorganization of cytoskeletal actin fiber structure in osteoblastic cells.
|
| ジャーナル |
J Biomech
|
| Abstract |
The cytoskeletal stress fiber structure plays essential roles in various kinds of cellular functions such as shape maintenance, active motility and mechanosensing, and its structure is dynamically reorganized under each functional process. In known reorganization mechanisms of the stress fibers, a change in its mechanical condition has been suggested as one of the key mediators that affect the reorganization process. Some experimental studies have clarified that tension release in the stress fibers induces fiber depolymerization that is considered to be the initial phase of the reorganization process. However, quantitative mechanical values such as strain or stress that induce depolymerization have still not been evaluated. This study is aimed at the quantitative evaluation of the mechanical value that induces stress fiber depolymerization, to gain a basic understanding of the reorganization phenomenon from a mechanical viewpoint. Osteoblastic cells (MC3T3-E1) were cultured on prestretched silicone rubber substrate. Compressive deformation was applied to the cells by uniaxially releasing the prestretched substrate strain and change in the stress fiber structure was observed. The results indicated that the compressive strain magnitude, not in the whole cell body but in the stress fiber itself, is important to induce disassembly of the stress fiber structure. The existence of a threshold strain magnitude for initiating fiber disassembly was also suggested; the threshold strain magnitude was evaluated as approximately -0.20.
|
| 巻・号 |
38(9)
|
| ページ |
1895-901
|
| 公開日 |
2005-9-1
|
| DOI |
10.1016/j.jbiomech.2004.08.012
|
| PII |
S0021-9290(04)00403-8
|
| PMID |
16023478
|
| MeSH |
Actin Cytoskeleton / metabolism*
Actin Cytoskeleton / ultrastructure*
Animals
Anisotropy
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods
Cytoskeleton / physiology*
Cytoskeleton / ultrastructure*
Differential Threshold / physiology
Elasticity
Equipment Design
Equipment Failure Analysis
Image Interpretation, Computer-Assisted
Mechanotransduction, Cellular / physiology*
Mice
NIH 3T3 Cells
Osteoblasts / cytology*
Osteoblasts / physiology*
Protein Conformation
Quantitative Structure-Activity Relationship
Stress, Mechanical
|
| IF |
2.32
|
| 引用数 |
49
|
|
WOS 分野
|
BIOPHYSICS
ENGINEERING, BIOMEDICAL
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
