| RRC ID |
1482
|
| 著者 |
Terakawa M, Tomimori Y, Goto M, Hayashi Y, Oikawa S, Fukuda Y.
|
| タイトル |
Eosinophil migration induced by mast cell chymase is mediated by extracellular signal-regulated kinase pathway.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Mast cell chymase is known to induce eosinophil migration in vivo and in vitro. In the present study, we investigated possible involvement of mitogen-activated protein (MAP) kinases; extracellular signal-regulated kinase (ERK), c-Jun amino-terminal kinase (JNK), and p38, in the chymase-induced eosinophil migration. Human chymase induced a rapid phosphorylation of ERK1/2 and p38 in human eosinophilic leukemia EoL-1 cells, while no phosphorylation was detected in JNK. The chymase-induced phosphorylation of ERK and p38 was inhibited by pertussis toxin. Similar results were obtained in the experiments using mouse chymase and eosinophils. U0126 (the inhibitor for MAP/ERK kinase) suppressed chymase-induced migration of EoL-1 cells and mouse eosinophils. However, SB203580 (p38 inhibitor) and SP600125 (JNK inhibitor) showed little effect on the migration. It is suggested therefore that chymase activates ERK and p38 probably through G-protein-coupled receptor, and that ERK but not p38 cascade may have a crucial role in chymase-induced migration of eosinophils.
|
| 巻・号 |
332(4)
|
| ページ |
969-75
|
| 公開日 |
2005-7-15
|
| DOI |
10.1016/j.bbrc.2005.04.172
|
| PII |
S0006-291X(05)00823-5
|
| PMID |
15919053
|
| MeSH |
Animals
Anthracenes / pharmacology
Blotting, Western
Butadienes / pharmacology
Butyric Acid / chemistry
Cell Line, Tumor
Cell Movement
Chemotaxis
Chymases
Enzyme Inhibitors / pharmacology
Eosinophils / cytology*
Eosinophils / metabolism*
Extracellular Signal-Regulated MAP Kinases / metabolism*
Humans
Imidazoles / pharmacology
JNK Mitogen-Activated Protein Kinases / metabolism
Mast Cells / enzymology*
Mice
Mice, Inbred BALB C
Mitogen-Activated Protein Kinase 3 / metabolism
Nitriles / pharmacology
Pertussis Toxin / pharmacology
Phosphorylation
Pyridines / pharmacology
Receptors, G-Protein-Coupled / metabolism
Serine Endopeptidases / chemistry*
Serine Endopeptidases / physiology
Signal Transduction
Time Factors
p38 Mitogen-Activated Protein Kinases / metabolism
|
| IF |
2.985
|
| 引用数 |
14
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
EoL-1 cell(RCB0641) |
