Reference - Detail
| RRC ID | 1502 |
|---|---|
| Author | Hansman GS, Natori K, Oka T, Ogawa S, Tanaka K, Nagata N, Ushijima H, Takeda N, Katayama K. |
| Title | Cross-reactivity among sapovirus recombinant capsid proteins. |
| Journal | Arch Virol |
| Abstract |
Sapovirus (SaV), a member of the genus Sapovirus in the family Caliciviridae, is an agent of human and porcine gastroenteritis. SaV strains are divided into five genogroups (GI-GV) based on their capsid (VP1) sequences. Human SaV strains are noncultivable, but expression of the recombinant capsid protein (rVP1) in a baculovirus expression system results in the self-assembly of virus-like particles (VLPs) that are morphologically similar to native SaV. In this study, rVP1 constructs of SaV GI, GII, and GV strains were expressed in a baculovirus expression system. The structures of the GI, GII, and GV VLPs, with diameters of 41-48 nm, were morphologically similar to those of native SaV. However a fraction of GV VLPs were smaller, with diameters of 26-31 nm and spikes on the outline. This is the first report of GII and GV VLP formation and the first identification of small VLPs. To examine the cross-reactivities among GI, GII, and GV rVP1, hyperimmune rabbit antisera were raised against Escherichia coli-expressed GI, GII, and GV N- and C-terminal VP1. Western blotting showed the GI antisera cross-reacted with GV rVP1 but not GII rVP1; GII antisera cross-reacted weakly with GI rVP1 but did not cross-react with GV rVP1; and GV antisera reacted only with GV rVP1. Also, hyperimmune rabbit and guinea pig antisera raised against purified GI VLPs were used to examine the cross-reactivities among GI, GII, and GV VLPs by an antigen enzyme-linked immunosorbent assay (ELISA). The ELISA showed that the GI VLPs were antigenically distinct from GII and GV VLPs. |
| Volume | 150(1) |
| Pages | 21-36 |
| Published | 2005-1-1 |
| DOI | 10.1007/s00705-004-0406-8 |
| PMID | 15449145 |
| MeSH | Animals Antibodies, Viral / immunology Baculoviridae / genetics* Baculoviridae / metabolism Caliciviridae / genetics Caliciviridae / immunology* Caliciviridae / metabolism Caliciviridae Infections / veterinary Caliciviridae Infections / virology Capsid / immunology* Capsid / metabolism Cloning, Molecular Cross Reactions Enzyme-Linked Immunosorbent Assay Guinea Pigs Molecular Sequence Data Phylogeny Rabbits Recombinant Proteins / immunology Spodoptera Virion / immunology* Virus Assembly |
| IF | 2.243 |
| Times Cited | 35 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Sf9(RCB0563) |