Reference - Detail
| RRC ID | 1512 |
|---|---|
| Author | Shirakawa J, Shibuya K, Shibuya A. |
| Title | Requirement of the serine at residue 329 for lipid raft recruitment of DNAM-1 (CD226). |
| Journal | Int Immunol |
| Abstract |
Upon antigen recognition by the TCR, leukocyte function-associated antigen-1 (LFA-1) physically associates with the leukocyte adhesion molecule DNAM-1 (CD226), for which the serine phosphorylation at residue 329 (S329) of DNAM-1 plays a critical role. The TCR-mediated signal also induces the formation of the immunological synapse (IS), in which lipid raft-associated molecules, including LFA-1, DNAM-1, protein kinase C, Fyn and others, are recruited, resulting in efficient signal transduction for T cell activation. However, the molecular mechanisms of lipid raft recruitment of many associated molecules have remained unclear. Here, we demonstrate that, while both wild-type (WT) and mutant DNAM-1 at S329 were polarized at the IS, the WT, but not mutant, DNAM-1 associated with lipid rafts at the peripheral supra-molecular activation clusters. We also demonstrate that the association of DNAM-1 with lipid rafts was necessary for the tyrosine phosphorylation of DNAM-1, which is essential for LFA-1-mediated co-stimulatory signaling for naive T cell proliferation and differentiation. |
| Volume | 17(3) |
| Pages | 217-23 |
| Published | 2005-3-1 |
| DOI | 10.1093/intimm/dxh199 |
| PII | dxh199 |
| PMID | 15684041 |
| MeSH | Antigens, Differentiation, T-Lymphocyte / chemistry Antigens, Differentiation, T-Lymphocyte / metabolism* Humans Lymphocyte Function-Associated Antigen-1 / metabolism Membrane Microdomains / metabolism* Phosphorylation Serine / chemistry Serine / metabolism* Signal Transduction |
| IF | 3.519 |
| Times Cited | 29 |
| WOS Category | IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAJI(RCB1647) |