| RRC ID |
1515
|
| Author |
Tsuji AB, Sudo H, Sugyo A, Otsuki M, Miyagishi M, Taira K, Imai T, Harada YN.
|
| Title |
A fast, simple method for screening radiation susceptibility genes by RNA interference.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes.
|
| Volume |
333(4)
|
| Pages |
1370-7
|
| Published |
2005-8-12
|
| DOI |
10.1016/j.bbrc.2005.06.047
|
| PII |
S0006-291X(05)01265-9
|
| PMID |
15979584
|
| MeSH |
Cell Count / methods*
Cell Survival / radiation effects*
Disease Susceptibility / diagnosis
Dose-Response Relationship, Drug
Gene Expression Profiling / methods*
Gene Silencing*
HeLa Cells
Humans
RNA Interference*
Radiation Dosage
Radiation Injuries / diagnosis
Radiation Injuries / etiology
Radiation Injuries / genetics*
Radiation Tolerance / genetics*
Radiation, Ionizing
Risk Assessment / methods*
Risk Factors
|
| IF |
2.985
|
| Times Cited |
11
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
