Reference - Detail
| RRC ID | 1515 |
|---|---|
| Author | Tsuji AB, Sudo H, Sugyo A, Otsuki M, Miyagishi M, Taira K, Imai T, Harada YN. |
| Title | A fast, simple method for screening radiation susceptibility genes by RNA interference. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes. |
| Volume | 333(4) |
| Pages | 1370-7 |
| Published | 2005-8-12 |
| DOI | 10.1016/j.bbrc.2005.06.047 |
| PII | S0006-291X(05)01265-9 |
| PMID | 15979584 |
| MeSH | Cell Count / methods* Cell Survival / radiation effects* Disease Susceptibility / diagnosis Dose-Response Relationship, Drug Gene Expression Profiling / methods* Gene Silencing* HeLa Cells Humans RNA Interference* Radiation Dosage Radiation Injuries / diagnosis Radiation Injuries / etiology Radiation Injuries / genetics* Radiation Tolerance / genetics* Radiation, Ionizing Risk Assessment / methods* Risk Factors |
| IF | 2.985 |
| Times Cited | 11 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |